{"product_id":"active-collagen-type-vi-alpha-1-col6a1-apc150mu03","title":"Active Collagen Type VI Alpha 1 (COL6a1)","description":"\u003cp\u003eProduct No.\u003c\/p\u003e\n\u003cp\u003eAPC150Mu03\u003c\/p\u003e\n\u003cp\u003eOrganism Species\u003c\/p\u003e\n\u003cp\u003eMus musculus (Mouse).\u003c\/p\u003e\n\u003cp\u003eBuffer Formulation\u003c\/p\u003e\n\u003cp\u003e20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.\u003c\/p\u003e\n\u003cp\u003eTraits\u003c\/p\u003e\n\u003cp\u003eFreeze-dried powder\u003c\/p\u003e\n\u003cp\u003ePurity\u003c\/p\u003e\n\u003cp\u003e\u0026gt; 95%\u003c\/p\u003e\n\u003cp\u003eIsoelectric Point\u003c\/p\u003e\n\u003cp\u003e6.3\u003c\/p\u003e\n\u003cp\u003eApplications\u003c\/p\u003e\n\u003cp\u003eCell culture; Activity Assays.\u003c\/p\u003e\n\u003cp\u003eUOM\u003c\/p\u003e\n\u003cp\u003e10µg 50µg 200µg 1mg 5mg\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product\"\u003e\n\u003cdiv class=\"product_left\"\u003e\n\u003ch3\u003eACTIVITY TEST\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003cdiv class=\"product_image\"\u003e\n\u003cimg src=\"https:\/\/www.cloud-clone.com\/upload\/product\/pictures\/APC150Mu03-201802271721107614.jpg\"\u003e\n\u003cp\u003eFigure. The binding activity of COL6a1 with RTBDN.\u003cbr\u003eThe collagens are a superfamily of proteins that play a role in maintaining the integrity of various tissues. Collagen VI is a major structural component of microfibrils. The basic structural unit of collagen VI is a heterotrimer of the alpha1(VI), alpha2(VI), and alpha3(VI) chains. The protein encoded by COL6a1 gene is the alpha 1 subunit of type VI collagen (alpha1(VI) chain). Mutations in the genes that code for the collagen VI subunits result in the autosomal dominant disorder, Bethlem myopathy. Besides, Retbindin (RTBDN) has been identified as an interactor of COL6a1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse COL6a1 and recombinant mouse RTBDN. Briefly, COL6a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to RTBDN-coated microtiter wells and incubated for 2h at 37℃. Wells were washed with PBST and incubated for 1h with anti-COL6a1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37℃. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of COL6a1 and RTBDN was shown in Figure 1, and this effect was in a dose dependent manner.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eUSAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eReconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg\/mL. Do not vortex.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTORAGE\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eAvoid repeated freeze\/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003ch3\u003eSTABILITY\u003c\/h3\u003e\n\u003cdiv\u003e\n\u003cp\u003eThe thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cp\u003e \u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_reference\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eMagazine\u003c\/td\u003e\n\u003ctd\u003eCitations\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular \u0026amp; Cellular Proteomics\u003c\/td\u003e\n\u003ctd\u003eIntegrated Proteomic Profiling of Cell Line Conditioned Media and Pancreatic Juice for the Identification of Pancreatic Cancer Biomarkers\u003cspan\u003e \u003c\/span\u003e\u003ca rel=\"nofollow\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3205865\/\" target=\"_blank\"\u003ePubMed: PMC3205865\u003c\/a\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"product_relation\"\u003e\n\u003ctable\u003e\n\u003ctbody\u003e\n\u003ctr class=\"first_tr\"\u003e\n\u003ctd class=\"td_1\"\u003eCatalog No.\u003c\/td\u003e\n\u003ctd class=\"td_2\"\u003eRelated products for research use of Mus musculus (Mouse) Organism species\u003c\/td\u003e\n\u003ctd class=\"td_3\"\u003eApplications (RESEARCH USE ONLY!)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPC150Mu03\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APC150Mu03.html\"\u003eActive Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPC150Mu02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPC150Mu02.html\"\u003eRecombinant Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPC150Mu03\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPC150Mu03.html\"\u003eRecombinant Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRPC150Mu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/RPC150Mu01.html\"\u003eRecombinant Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003ePositive Control; Immunogen; SDS-PAGE; WB.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAPC150Mu02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/APC150Mu02.html\"\u003eActive Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eCell culture; Activity Assays.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAC150Mu01\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAC150Mu01.html\"\u003ePolyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAC150Mu03\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAC150Mu03.html\"\u003ePolyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePAC150Mu02\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/PAC150Mu02.html\"\u003ePolyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC; IP.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLAC150Mu71\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LAC150Mu71.html\"\u003eBiotin-Linked Polyclonal Antibody to Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eWB; IHC; ICC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSEC150Mu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/SEC150Mu.html\"\u003eELISA Kit for Collagen Type VI Alpha 1 (COL6a1)\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eLMC150Mu\u003c\/td\u003e\n\u003ctd\u003e\u003ca href=\"https:\/\/www.cloud-clone.com\/products\/LMC150Mu.html\"\u003eMagnetic Luminex Assay Kit for Collagen Type VI Alpha 1 (COL6a1) ,etc.\u003c\/a\u003e\u003c\/td\u003e\n\u003ctd\u003eMagnetic Luminex Assay for Antigen Detection.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv id=\"more_info\"\u003e\u003c\/div\u003e","brand":"Cloud-clone","offers":[{"title":"50ug \/ \u003e 95% \/ Mus musculus (Mouse)","offer_id":41485887013088,"sku":"APC150Mu03","price":774.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/products\/NP_74353e22-4da5-487b-985e-8a771fffa2ab.jpg?v=1633607250","url":"https:\/\/rndmate.com\/products\/active-collagen-type-vi-alpha-1-col6a1-apc150mu03","provider":"알앤디메이트","version":"1.0","type":"link"}