{"product_id":"anti-creb-h-creb3l3-antibody","title":"Anti-CREB-H (CREB3L3) Antibody","description":"\u003cdiv class=\"pro-tab-box mobile-tabview pull-left padlr-no padtb-10 col-xs-12\"\u003e\n\u003cdiv class=\"w100 pull-left ol-references\"\u003e\n\u003col\u003e\n\u003cli\u003e\n\u003cp\u003e\u003cstrong\u003eThis rabbit IgG polyclonal antibody was generated against purified protein segment, N-terminal amino acids 75 to 250 of mouse cAMP-responsive element binding protein, hepatocyte specific (CREB-H) protein and reacts against human and mouse CREB-H.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHighlights\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul\u003e\n\u003cli\u003eExhibits high specificity and high titer\u003c\/li\u003e\n\u003cli\u003eReacts with human and mouse CREBH\u003c\/li\u003e\n\u003cli\u003eSuitable for Western Blot, Immunoprecipitation and ChIP analysis applications\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eCREB-H (also called CREB3L3) is a stress sensor that plays key roles in lipid and glucose metabolism associated with metabolic disease. CREB-H is a liver-specific bZIP transcription factor with molecular weights of 52.7 kD (precursor predicted size) and 35.3 kD (activated form predicted size). The CREB-H protein is localized to the endoplasmic reticulum and acts as a transcription factor activated by cyclic AMP stimulation.\u003c\/p\u003e\n\u003cp class=\"italic\"\u003eFrom the laboratory of\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.kerafast.com\/s-434-kezhong-zhang-phd.aspx\"\u003eKezhong Zhang, PhD\u003c\/a\u003e, Wayne State University.\u003c\/p\u003e\n\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cdiv class=\"pro-tab-box mobile-tabview pull-left padlr-no padtb-10 col-xs-12\"\u003e\n\u003cdiv id=\"tabcontspecification\" class=\"ptabs-heads mobile-tab-1 mb-15\"\u003eSPECIFICATIONS\u003c\/div\u003e\n\u003cdiv class=\"ptabs-cont mobile-tab-cont pull-left\"\u003e\n\u003ctable class=\"pro-spect-table\" width=\"100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eProduct Type:\u003c\/td\u003e\n\u003ctd\u003eAntibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAntigen:\u003c\/td\u003e\n\u003ctd\u003eCREB-H, CREB3L3\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAccession ID:\u003c\/td\u003e\n\u003ctd\u003eQ68CJ9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMolecular Weight:\u003c\/td\u003e\n\u003ctd\u003e52.7 kDa (predicted)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eIsotype:\u003c\/td\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eClonality:\u003c\/td\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eReactivity:\u003c\/td\u003e\n\u003ctd\u003eHuman and mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eImmunogen:\u003c\/td\u003e\n\u003ctd\u003ePurified protein segment, N-terminal 176 amino acids\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpecies Immunized:\u003c\/td\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEpitope:\u003c\/td\u003e\n\u003ctd\u003eAA 75 - 250 of CREB-H protein\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003ePurification Method:\u003c\/td\u003e\n\u003ctd\u003eAffinity Purified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer:\u003c\/td\u003e\n\u003ctd\u003ePBS\/0.05% Azide, 50% Glycerol\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eTested Applications:\u003c\/td\u003e\n\u003ctd\u003eWB (1:1000-2000), IP-Western (1:1000), ChIP (1:1000)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eStorage:\u003c\/td\u003e\n\u003ctd\u003e-20C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eShipped:\u003c\/td\u003e\n\u003ctd\u003eCold packs\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pro-tab-box mobile-tabview pull-left padlr-no padtb-10 col-xs-12\"\u003e\n\u003cdiv id=\"tabDescription\" class=\"ptabs-heads mobile-tab-1 mb-15\"\u003eDOCUMENTATION\u003c\/div\u003e\n\u003cdiv class=\"w100 pull-left SEE-tabDescription\"\u003e\n\u003ch3\u003eNotes\u003c\/h3\u003e\n\u003cp\u003eFor Western blot analysis with total raw lysates from liver tissues, the antibody can detect an unspecific signal around 90 kD when films are incubated with PDVF membranes for a relatively long time. But the unspecific signal can be easily separated or distinguished from the specific signals of CREB-H precursor (around 75 kD gel size) and activated (around 50 kD gel size) forms.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pro-tab-box mobile-tabview pull-left padlr-no padtb-10 col-xs-12\"\u003e\n\u003cdiv id=\"tabData\" class=\"ptabs-heads mobile-tab-1 mb-15\"\u003eDATA\u003c\/div\u003e\n\u003cdiv class=\"w100 pull-left tabData\"\u003e\n\u003cp\u003e\u003cstrong\u003eIP-Western Blot Analysis\u003c\/strong\u003e\u003c\/p\u003e\n\u003cdiv\u003e\u003cimg src=\"https:\/\/www.kerafast.com\/Images\/Zhangdata.jpg\" height=\"300\" width=\"424\" alt=\"WB Data\"\u003e\u003c\/div\u003e\n\u003cp\u003eIP-Western blot analysis of CREB-H and PPAR? interaction in the livers of mice under normal chow, after 14-hour fasting, or after 6 months of AHF diet. Whole-protein lysates were prepared from mouse liver tissues and subjected to IP to pull down the endogenous PPAR? or CREB-H protein complex using an anti-PPAR? or anti-CREB-H antibody. The precipitates were analyzed by immunoblotting with the antibody against CREB-H or PPAR?. Whole-liver protein lysates were subjected to Western blot analysis to determine the levels of CREB-H, PPAR?, and ?-actin as the controls. The values below the gels represent the interactive CREB-H (with PPAR?) and PPAR? (with CREB-H) protein signal intensities after normalization to ?-actin signal intensities. Ab, antibody; Ad, adenovirus.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAdapted from: \u003c\/strong\u003eKim, H., et al. Endocrinology 155: 769-782. 2014 Mar;155(3):769-82.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pro-tab-box mobile-tabview pull-left padlr-no padtb-10 col-xs-12\"\u003e\n\u003cdiv id=\"tabProvider\" class=\"ptabs-heads mobile-tab-1 mb-15\"\u003ePROVIDER\u003c\/div\u003e\n\u003cdiv class=\"w100 pull-left tabProvider\"\u003eFrom the laboratory of\u003cspan\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.kerafast.com\/s-434-kezhong-zhang-phd.aspx\"\u003eKezhong Zhang, PhD\u003c\/a\u003e, Wayne State University.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"pro-tab-box mobile-tabview pull-left padlr-no padtb-10 col-xs-12\"\u003e\n\u003cdiv id=\"tabreferences\" class=\"ptabs-heads mobile-tab-1 mb-15\"\u003eREFERENCES\u003c\/div\u003e\n\u003cdiv class=\"w100 pull-left ol-references\"\u003e\n\u003col\u003e\n\u003cli\u003eKim, H., Mendez, R., Zheng, Z., Chang, L., Cai, J., Zhang, R., and Zhang, K. 2014. Liver-enriched Transcription Factor CREBH Interacts with Peroxisome Proliferator-activated Receptor ? to Regulate Metabolic Hormone FGF21. Endocrinology 155: 769-782. 2014 Mar;155(3):769-82\u003c\/li\u003e\n\u003cli\u003eZhang, C., Wang, G., Zheng, Z., Maddipati, K.R., Zhang, X., Dyson, G., Williams, P., Duncan, S.A., Kaufman, R.J., and Zhang, K. 2012. ER-tethered Transcription Factor CREBH Regulates Hepatic Lipogenesis, Fatty Acid Oxidation, and Lipolysis upon Metabolic Stress. Hepatology 55 (4): 1070-1082. PMCID: PMC3319338.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"Kerafast","offers":[{"title":"Default Title","offer_id":44868205314272,"sku":"EWS101","price":0.0,"currency_code":"KRW","in_stock":true}],"url":"https:\/\/rndmate.com\/products\/anti-creb-h-creb3l3-antibody","provider":"알앤디메이트","version":"1.0","type":"link"}