{"product_id":"antibodysystem-ehj24002","title":"Recombinant Human PCSK9 (Asp374Tyr) Protein, C-His","description":"\u003cp\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003e: EHJ24002\u003c\/p\u003e\u003cp\u003eGenetic variation in PCSK9 has an enormous impact on LDL-C concentration in humans and both gain-of-function (GOF) and loss-of-function (LOF) PCSK9 mutations have been described. While PCSK9 LOF mutations cause hypocholesterolemia, GOF mutations are a rare cause of familial hypercholesterolemia (FH), a monogenic disease characterized by very high levels of LDL-C and premature atherosclerotic cardiovascular disease (ASCVD). PCSK9 GOF mutations are causative of FH, because the enhancement in PCSK9 function leads to increased LDLr degradation and reduced recycling to the cell surface. As a consequence, there is a reduction in LDL uptake and an increase in circulating LDL-C concentration. The best characterized PCSK9 GOF mutation is p.(Asp374Tyr) which produces a ten-fold increase in LDLr degradation by increasing the binding affinity of PCSK9 to the epidermal growth factor-like domain of LDLr. This variant was demonstrated to inhibit LDL uptake still at a concentration 25 times lower than the wild-type PCSK9.\u003c\/p\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog No.\u003c\/th\u003e\n\u003ctd\u003eEHJ24002\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct name\u003c\/th\u003e\n\u003ctd\u003eRecombinant Human PCSK9 (Asp374Tyr) Protein, C-His\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100 μg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eExpression system\u003c\/th\u003e\n\u003ctd\u003eMammalian Cells\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProtein length\u003c\/th\u003e\n\u003ctd\u003eMet1-Gln692 (D374Y)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePredicted molecular weight\u003c\/th\u003e\n\u003ctd\u003e75.30 kDa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAccession\u003c\/th\u003e\n\u003ctd\u003eQ8NBP7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003ctd\u003eGenetic variation in PCSK9 has an enormous impact on LDL-C concentration in humans and both gain-of-function (GOF) and loss-of-function (LOF) PCSK9 mutations have been described. While PCSK9 LOF mutations cause hypocholesterolemia, GOF mutations are a rare cause of familial hypercholesterolemia (FH), a monogenic disease characterized by very high levels of LDL-C and premature atherosclerotic cardiovascular disease (ASCVD). PCSK9 GOF mutations are causative of FH, because the enhancement in PCSK9 function leads to increased LDLr degradation and reduced recycling to the cell surface. As a consequence, there is a reduction in LDL uptake and an increase in circulating LDL-C concentration. The best characterized PCSK9 GOF mutation is p.(Asp374Tyr) which produces a ten-fold increase in LDLr degradation by increasing the binding affinity of PCSK9 to the epidermal growth factor-like domain of LDLr. This variant was demonstrated to inhibit LDL uptake still at a concentration 25 times lower than the wild-type PCSK9.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLyophilized\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage buffer\u003c\/th\u003e\n\u003ctd\u003eLyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;90% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003ePlease contact with the lab for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNature\u003c\/th\u003e\n\u003ctd\u003eRecombinant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStability and Storage\u003c\/th\u003e\n\u003ctd\u003eUse a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReconstitution\u003c\/th\u003e\n\u003ctd\u003eReconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative Names\u003c\/th\u003e\n\u003ctd\u003eProprotein convertase subtilisin\/kexin type 9, Subtilisin\/kexin-like protease PC9, NARC-1, PC9, Proprotein convertase 9, Neural apoptosis-regulated convertase 1, NARC1, PCSK9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eHomo sapiens (Human)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eIn general, proteins are provided as lyophilized powder\/frozen liquid. They are shipped out with dry ice\/blue ice unless customers require otherwise.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNote\u003c\/th\u003e\n\u003ctd\u003eFor research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"AntibodySystem","offers":[{"title":"Default Title","offer_id":51485242949856,"sku":"EHJ24002","price":480.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/AntibodySystem_64e7be99-7d13-4d50-a5d0-3fbb1d13b943.png?v=1776221083","url":"https:\/\/rndmate.com\/products\/antibodysystem-ehj24002","provider":"알앤디메이트","version":"1.0","type":"link"}