{"product_id":"antibodysystem-yxx26701","title":"Lba Cas12a (Cpf1) Nuclease","description":"\u003cp\u003e\u003cstrong\u003eCatalog No.\u003c\/strong\u003e: YXX26701\u003c\/p\u003e\u003cp\u003eLba Cas12a nuclease is derived from Lachnospiraceae bacterium ND2006 and is a site-specific DNA endonuclease mediated by a single-guide RNA (gRNA) with a length of 41-44 nucleotides. In the presence of a PAM (TTTN) sequence on the target double-stranded DNA, LbCas12a specifically cleaves the target dsDNA, resulting in double-strand breaks and the generation of sticky ends. In contrast, the specific cleavage of single-stranded DNA targets by LbCas12a does not require a PAM sequence. \\r\\n\u003cbr\u003e\u003cbr\u003eLba Cas12a contains simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N-terminus and C-terminus. When Cas12a is expressed with these NLS sequences, the Cas12a-RNP complex can rapidly localize to the nucleus upon cellular entry. \\r\\n\u003cbr\u003e\u003cbr\u003eAdditionally, both double-stranded and single-stranded DNA targets can activate the trans-cleavage activity of LbCas12a. Specifically, upon formation of a ternary complex among the LbCas12a enzyme, crRNA, and target DNA, the enzyme becomes activated and exhibits non-specific trans-cleavage activity, degrading any single-stranded DNA (ssDNA) present in the system. Consequently, LbCas12a can not only be used for specific cleavage of dsDNA in vitro but also enables rapid detection of target nucleic acids.\u003c\/p\u003e\u003ctable\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eCatalog No.\u003c\/th\u003e\n\u003ctd\u003eYXX26701\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct name\u003c\/th\u003e\n\u003ctd\u003eLba Cas12a (Cpf1) Nuclease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e200 μl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDescription\u003c\/th\u003e\n\u003ctd\u003eLba Cas12a nuclease is derived from Lachnospiraceae bacterium ND2006 and is a site-specific DNA endonuclease mediated by a single-guide RNA (gRNA) with a length of 41-44 nucleotides. In the presence of a PAM (TTTN) sequence on the target double-stranded DNA, LbCas12a specifically cleaves the target dsDNA, resulting in double-strand breaks and the generation of sticky ends. In contrast, the specific cleavage of single-stranded DNA targets by LbCas12a does not require a PAM sequence. \\r\\n\u003cbr\u003e\u003cbr\u003eLba Cas12a contains simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N-terminus and C-terminus. When Cas12a is expressed with these NLS sequences, the Cas12a-RNP complex can rapidly localize to the nucleus upon cellular entry. \\r\\n\u003cbr\u003e\u003cbr\u003eAdditionally, both double-stranded and single-stranded DNA targets can activate the trans-cleavage activity of LbCas12a. Specifically, upon formation of a ternary complex among the LbCas12a enzyme, crRNA, and target DNA, the enzyme becomes activated and exhibits non-specific trans-cleavage activity, degrading any single-stranded DNA (ssDNA) present in the system. Consequently, LbCas12a can not only be used for specific cleavage of dsDNA in vitro but also enables rapid detection of target nucleic acids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity\u003c\/th\u003e\n\u003ctd\u003e\u0026gt;90% as determined by SDS-PAGE.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eApplications\u003c\/th\u003e\n\u003ctd\u003eGenome Editing, DNA Detection, CRISPR Diagnostics\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin level\u003c\/th\u003e\n\u003ctd\u003ePlease contact with the lab for this information.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNature\u003c\/th\u003e\n\u003ctd\u003eRecombinant\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStability and Storage\u003c\/th\u003e\n\u003ctd\u003eStore at -20°C for twelve months from the date of receipt.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAlternative Names\u003c\/th\u003e\n\u003ctd\u003eCRISPR-associated endonuclease Cas12a, CRISPR-associated endonuclease Cpf1, Lba Cas12a (Cpf1), LbaCas12a, Cpf1, LbCas12a\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies\u003c\/th\u003e\n\u003ctd\u003eLachnospiraceae bacterium ND2006\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShipping\u003c\/th\u003e\n\u003ctd\u003eIn general, proteins are provided as lyophilized powder\/frozen liquid. They are shipped out with dry ice\/blue ice unless customers require otherwise.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNote\u003c\/th\u003e\n\u003ctd\u003eFor research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e","brand":"AntibodySystem","offers":[{"title":"Default Title","offer_id":51490785165536,"sku":"YXX26701","price":770.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/AntibodySystem_bb5d7d68-2179-4095-9130-e2c547c1ec3d.png?v=1776227070","url":"https:\/\/rndmate.com\/products\/antibodysystem-yxx26701","provider":"알앤디메이트","version":"1.0","type":"link"}