{"product_id":"bek1199","title":"Human Survivin ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of Surviv in in human serum,plasma, body fluids, tissue lysates or cell culture supernates.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Survivin, also called baculoviral inhibitor of apoptosis repeat-containing 5 or BIRC5, is an inhibitor of apoptosis (IAP), or programmed cell death, that is selectively overexpressed in common human cancers.Itisthe smallestmember of the inhibitor of apoptosis (IAP) family. It is expressed highly in most human tumours and fetal tissue, but is completely absent in terminally differentiated cells. The survivin can inhibit caspase activation, thereby leading to negative regulation of apoptosis or programmed cell death. The overexpression of survivin in tumors may serve to increase the tumors resistance to apoptosis and, thus, contribute to cell immortality even in the presence of death stimuli.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Survivin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Survivin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Survivin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Survivin can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 62.5 pg\/ml - 4000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 2 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":47539421151456,"sku":"BEK1199","price":500.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/Biospes-logo_991b9317-3e32-4055-9209-f1c679d3148a.jpg?v=1750988863","url":"https:\/\/rndmate.com\/products\/bek1199","provider":"알앤디메이트","version":"1.0","type":"link"}