{"product_id":"bek1203","title":"Rat TGFβ1 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of TGFβ1 in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Transforming growth factor beta 1 or TGFβ1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. In human s, TGFβ1 is encoded by the TGFB1 gene. This gene contains 7 exons and very large introns, maps to 19q13.1-q13.3. TGFβ1 acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. The TGFB1 is directly involved in the pathogenesis of bone marrow reticulin fibrosis in hairy cell leukemia. The expression of TGFB1 in the early stages of DMD may be critical in initiating muscle fibrosis, and suggested that antifibrosis treatment might slow progression of the disease, increasing the utility of gene therapy.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TGFβ1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TGFβ1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theTGFβ1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofTGFβ1can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 15.6 pg\/ml - 1000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 3.5 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":47539421577440,"sku":"BEK1203","price":500.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/Biospes-logo_ac321f94-3a43-4754-85bd-aa40f0b1e2b7.jpg?v=1750988874","url":"https:\/\/rndmate.com\/products\/bek1203","provider":"알앤디메이트","version":"1.0","type":"link"}