{"product_id":"bek1216","title":"Human Soluble TNFR I, sTNFR I ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of sTNFR I in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNFɑ).Tumor necrosis factor receptor superfamily member 1A is a protein that in human s is encoded by the TNFRSF1A gene, which chromosome 12p13.2. It is a member of the Tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. Chan et al. (2000) found that, in contrast, the p60 and p80 TNFA receptors self-assemble through a distinct functional domain in the TNFR extracellular domain, termed the pre-ligand assembly domain (PLAD), in the absence of ligand.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-sTNFR I polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-sTNFR I polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction.TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the sTNFR I amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of sTNFR I can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 7.8 pg\/ml - 500 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 1 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":47539422789856,"sku":"BEK1216","price":500.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/Biospes-logo_89dc4ea7-18e2-444f-81be-cb7fd8300e84.jpg?v=1750988910","url":"https:\/\/rndmate.com\/products\/bek1216","provider":"알앤디메이트","version":"1.0","type":"link"}