{"product_id":"bek1299","title":"Human ACVRL1\/ALK-1 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of ACVRL1 \/ ALK-1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Serine\/threonine-protein kinase receptor R3 is an enzyme that in human s is encoded by the ACVRL1 gene.ACVRL1 is a receptor in the TGF beta signaling pathway. It is also known as activin receptor-like kinase 1, or ALK1.This gene encodes a type I cell-surface receptor for the TGF-beta superfamily of ligands. It shares with other type I receptors a high degree of similarity in serine-threonine kinase subdomains, a glycine- and serine-rich region (called the GS domain) preceding the kinase domain, and a short C-terminal tail. The encoded protein, sometimes termed ALK1, shares similar domain structures with other closely related ALK or activin receptor-like kinase proteins that form a subfamily of receptor serine\/threonine kinases.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-ACVRL1 \/ ALK-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-ACVRL1 \/ ALK-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the ACVRL1 \/ ALK-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of ACVRL1 \/ ALK-1 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 46.9 pg\/ml - 3000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 15 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":47539430686944,"sku":"BEK1299","price":500.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/Biospes-logo_cd3f95c8-b144-46b5-8e8a-9d87fec383cc.jpg?v=1750989105","url":"https:\/\/rndmate.com\/products\/bek1299","provider":"알앤디메이트","version":"1.0","type":"link"}