{"product_id":"bevacizumab-avastin-pharmacokinetic-elisa-el-1611-021","title":"Bevacizumab (Avastin) Pharmacokinetic ELISA","description":"\u003ch2\u003eBevacizumab (Avastin) Pharmacokinetic ELISA\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffinityImmuno Bevacizumab (Avastin) Pharmacokinetic ELISA\u003c\/strong\u003e is a ready-to-use ELISA kit designed for pharmacokinetic (PK), anti-drug antibody (ADA), and immunogenicity workflows in biologics and biosimilar development.\u003c\/p\u003e\n\u003cp\u003eBevacizumab (Trade name Avastin®) is a recombinant humanized monoclonal antibody that blocks angiogenesis by inhibiting vascular endothelial growth factor A (VEGF-A). VEGF-A stimulates angiogenesis in a variety of diseases, including cancer. Bevacizumab was the first clinically available angiogenesis inhibitor in the United States. Bevacizumab was approved by the U.S. Food and Drug Administration (FDA) for certain metastatic cancers. It received its first approval in 2004, for combined use with standard chemotherapy for metastatic colon cancer. It has since been approved for use in certain lung cancers, renal cancers, ovarian cancers, and glioblastoma multiforme of the brain. It had been approved for breast cancer, but that approval was withdrawn when later studies showed no evidence of effectiveness\u003c\/p\u003e\n\u003ch3\u003eProduct Highlights\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget Drug:\u003c\/strong\u003e Bevacizumab (Avastin)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct Type:\u003c\/strong\u003e ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplication:\u003c\/strong\u003e Quantification of Bevacizumab in biological matrices\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMethod:\u003c\/strong\u003e Direct sandwich ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Method:\u003c\/strong\u003e Peroxidase \/ OD450\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human, mouse, rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Range:\u003c\/strong\u003e 50ng\/ml - 1.56ng\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Minimum:\u003c\/strong\u003e 1.5ng\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Type:\u003c\/strong\u003e Serum Plasma\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Volume:\u003c\/strong\u003e 15ul\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Time:\u003c\/strong\u003e 2.5 hours\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e -20C, 1 year\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e dry ice, less than -20C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePack Size:\u003c\/strong\u003e 1X96 wells\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e Bevacizumab\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlate Format:\u003c\/strong\u003e Strip\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Details\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eComponents:\u003c\/strong\u003e Coated microtiter plate, 96 wells\nCalibrator diluent. - 1.8ml\nCalibrator 12ul\n10X wash buffer - 25ml\nAssay buffer - 50ml\n1000X detection reagent - 17ul\nTMB - 12ml\nTMB stop solution - 12ml\nPlate sealers - 3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Procedure:\u003c\/strong\u003e This assay employs the sandwich enzyme immunoassay technique. Anti- Bevacizumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Bevacizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Bevacizumab present in test samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReagent Preparation:\u003c\/strong\u003e Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation: Dilute wash buffer concentrate with ultra-pure water 1\/10 before use (for example add 20mL concentrate to 180mL ultra-pure water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1\/1000 before use (for example add 11μl concentrate to 11ml of assay buffer). Mix well. 3. Preparation of Calibrators: Prepare calibrators with concentrations ranging from 2,500 ng\/mL to 78 ng\/mL. The following is an example calibrator curve.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResults Calculation:\u003c\/strong\u003e 1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used. 2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample. 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with calibrator diluent and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Collection:\u003c\/strong\u003e This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Preparation:\u003c\/strong\u003e Dilute calibrators and test samples 1\/50 with assay buffer (for example add 5µL of prepared calibrator or sample to 245µL of assay buffer). Mix well. Do not store diluted samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Precision:\u003c\/strong\u003e \u0026lt;10%, \u0026lt;10%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePreservative:\u003c\/strong\u003e None\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGene ID:\u003c\/strong\u003e 7422\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eSEO Keywords:\u003c\/strong\u003e Bevacizumab (Avastin), Bevacizumab (Avastin) Pharmacokinetic ELISA, AffinityImmuno, ELISA, PK assay, ADA assay, biologics analysis, immunogenicity, bioanalytical testing, pharmacokinetic ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDatasheet:\u003c\/strong\u003e \u003ca href=\"http:\/\/affinityimmuno.com\/wp-content\/uploads\/2016\/10\/EL-1611-021-Avastin-PK-PRODUCT-SHEET.pdf\"\u003ehttp:\/\/affinityimmuno.com\/wp-content\/uploads\/2016\/10\/EL-1611-021-Avastin-PK-PRODUCT-SHEET.pdf\u003c\/a\u003e\u003c\/p\u003e","brand":"Affinity Immuno","offers":[{"title":"Default Title","offer_id":50127971942624,"sku":"EL-1611-021","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/AffinityImmuno-Product-3.jpg?v=1773910659","url":"https:\/\/rndmate.com\/products\/bevacizumab-avastin-pharmacokinetic-elisa-el-1611-021","provider":"알앤디메이트","version":"1.0","type":"link"}