{"product_id":"col2-elisa-kit","title":"COL2 ELISA Kit","description":"\u003cul class=\"accordion list-group list-group-flush\"\u003e\n\u003cli id=\"section-product_detail\" class=\"list-group-item list-group-item-action px-2 px-sm-0\"\u003e\n\u003cdl id=\"productDetailsproduct_detail\" class=\"collapse show\"\u003e\n\u003cdt\u003eTarget\u003c\/dt\u003e\n\u003cdd\u003eCOL2 (Collagen, Type II (COL2))\u003c\/dd\u003e\n\u003cdt\u003eReactivity\u003cspan\u003e \u003c\/span\u003e\n\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eMouse\u003c\/dd\u003e\n\u003cdt\u003eDetection Method\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eColorimetric\u003c\/dd\u003e\n\u003cdt\u003eMethod Type\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eSandwich ELISA\u003c\/dd\u003e\n\u003cdt\u003eDetection Range\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e62.5 pg\/mL - 4000 pg\/mL\u003c\/dd\u003e\n\u003cdt\u003eMinimum Detection Limit\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e62.5 pg\/mL\u003c\/dd\u003e\n\u003cdt\u003eApplication\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eELISA\u003c\/dd\u003e\n\u003cdt\u003ePurpose\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eThe kit is a sandwich enzyme immunoassay technique for the in vitro quantitative measurement in various sample types.\u003c\/dd\u003e\n\u003cdt\u003eSample Type\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eCell Culture Supernatant, Plasma, Serum\u003c\/dd\u003e\n\u003cdt\u003eAnalytical Method\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eQuantitative\u003c\/dd\u003e\n\u003cdt\u003eSpecificity\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eThis kit recognizes Mouse COL2 in samples. No Significant cross-reactivity or interference between Mouse COL2 and analogues was observed.\u003c\/dd\u003e\n\u003cdt\u003eSensitivity\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e37.5 pg\/mL\u003c\/dd\u003e\n\u003cdt\u003eComponents\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e\n\u003cul\u003e\n\u003cli\u003ePre-coated, ready to use 96-well strip plate, flat buttom\u003c\/li\u003e\n\u003cli\u003ePlate sealer for 96 wells\u003c\/li\u003e\n\u003cli\u003eReference Standard\u003c\/li\u003e\n\u003cli\u003eReference Standard \u0026amp; Sample Diluent\u003c\/li\u003e\n\u003cli\u003eBiotinylated Detection Antibody (100 x concentrate)\u003c\/li\u003e\n\u003cli\u003eHRP Conjugate (100 x concentrate)\u003c\/li\u003e\n\u003cli\u003eBiotinylated Detection Antibody Diluent\u003c\/li\u003e\n\u003cli\u003eHRP Conjugate Diluent\u003c\/li\u003e\n\u003cli\u003eSubstrate Reagent\u003c\/li\u003e\n\u003cli\u003eStop Solution\u003c\/li\u003e\n\u003cli\u003eWash Buffer (25 x concentrate)\u003c\/li\u003e\n\u003cli\u003eInstruction manual\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/dd\u003e\n\u003cdd\u003e\u003cbr\u003e\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003c\/li\u003e\n\u003cli id=\"section-application\" class=\"list-group-item list-group-item-action px-2 px-sm-0\"\u003e\n\u003cdiv class=\"mb-0\"\u003e\n\u003ch2 class=\"h4 fs-4 d-inline-block collapse-trigger\"\u003eApplication Details\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdl id=\"productDetailsapplication\" class=\"collapse show\"\u003e\n\u003cdt\u003eSample Volume\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e100 μL\u003c\/dd\u003e\n\u003cdt\u003eAssay Time\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e3.5 h\u003c\/dd\u003e\n\u003cdt\u003ePlate\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003ePre-coated\u003c\/dd\u003e\n\u003cdt\u003eProtocol\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e\n\u003col\u003e\n\u003cli\u003eAdd 100 µL standard or sample to each well. Incubate for 90 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eRemove the liquid. Add 100 µL Biotinylated Detection Antibody. Incubate for 1 hour at 37 °C.\u003c\/li\u003e\n\u003cli\u003eAspirate and wash 3 times.\u003c\/li\u003e\n\u003cli\u003eAdd 100 µL HRP Conjugate. Incubate for 30 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eAspirate and wash 5 times.\u003c\/li\u003e\n\u003cli\u003eAdd 90 µL Substrate Reagent. Incubate for 15 min at 37 °C.\u003c\/li\u003e\n\u003cli\u003eAdd 50 µL Stop Solution. Read at 450 nm immediately.\u003c\/li\u003e\n\u003cli\u003eCalculation of results.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/dd\u003e\n\u003cdt\u003eReagent Preparation\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e\n\u003col\u003e\n\u003cli\u003eBring all reagents to room temperature (18~25 °C) before use. Follow the Microplate reader manual for set-up and preheat it for 15 min before OD measurement.\u003c\/li\u003e\n\u003cli\u003eWash Buffer: Dilute 30 mL of Concentrated Wash Buffer with 720 mL of deionized or distilled water to prepare 750 mL of Wash Buffer.Note: if crystals have formed in the concentrate, warm it in a 40 °C water bath and mix it gently until the crystals have completely dissolved\u003c\/li\u003e\n\u003cli\u003eStandard working solution: Centrifuge the standard at 10,000xg for 1 min. Add 1.0 mL of Reference Standard \u0026amp;Sample Diluent, let it stand for 10 min and invert it gently several times. After it dissolves fully, mix it thoroughly with a pipette. This reconstitution produces a working solution of 4000 pg\/mL. Then make serial dilutions as needed. The recommended dilution gradient is as follows: 4000, 2000, 1000, 500, 250, 125, 62.50, 0 pg\/mL. Dilution method: Take 7 EP tubes, add 500 μLof Reference Standard \u0026amp; Sample Diluent to each tube. Pipette 500 μLof the 4000 pg\/mL working solution to the first tube and mix up to produce a 2000 pg\/mL working solution. Pipette 500 μLof the solution from the former tube into the latter one according to these steps. The illustration below is for reference. Note: the last tube is regarded as a blank. Don't pipette solution into it from the former tube.\u003c\/li\u003e\n\u003cli\u003eBiotinylated Detection Antibody working solution: Calculate the required amount before the experiment (100 μL\/well). In preparation, slightly more than calculated should be prepared. Centrifuge the stock tube before use, dilute the 100x Concentrated Biotinylated Detection Antibody to 1xworking solution with Biotinylated Detection Antibody Diluent.\u003c\/li\u003e\n\u003cli\u003eConcentrated HRP Conjugate working solution: Calculate the required amount before the experiment (100 μL\/well). In preparation, slightly more than calculated should be prepared. Dilute the 100x Concentrated HRP Conjugate to 1x working solution with Concentrated HRP Conjugate Diluent.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/dd\u003e\n\u003cdt\u003eSample Preparation\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e\n\u003cul\u003e\n\u003cli\u003eIt is recommended to use fresh samples without long storage, otherwise protein degradation and denaturationmay occur in these samples, leading to false results. Samples should therefore be stored for a short periodat 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thawcycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged toremove precipitates.\u003c\/li\u003e\n\u003cli\u003eIf the sample type is not specified in the instructions, a preliminary test is necessary to determinecompatibility with the kit.\u003c\/li\u003e\n\u003cli\u003eIf a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibilityof causing a deviation due to the introduced chemical substance.The recommended dilution factor is for reference only.\u003c\/li\u003e\n\u003cli\u003ePlease estimate the concentration of the samples before performing the test. If the values are not in therange of the standard curve, the optimal sample dilution for the particular experiment has to be determined.Samples should then be diluted with PBS (pH =7.0-7.2).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/dd\u003e\n\u003cdt\u003eAssay Precision\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eIntra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse COL2 were tested 20 times on one plate, respectively.\u003cbr\u003eInter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse COL2 were tested on 3 different plates, 20 replicates in each plate.\u003cbr\u003eBoth intra-CV and inter-CV are \u0026lt; 10 %.\u003c\/dd\u003e\n\u003cdt\u003eRestrictions\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eFor Research Use only\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003c\/li\u003e\n\u003cli id=\"section-storage_and_handling\" class=\"list-group-item list-group-item-action px-2 px-sm-0\"\u003e\n\u003cdiv class=\"mb-0\"\u003e\n\u003ch2 class=\"h4 fs-4 d-inline-block collapse-trigger\"\u003eHandling\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdl id=\"productDetailsstorage_and_handling\" class=\"collapse show\"\u003e\n\u003cdt\u003eStorage\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e4 °C,-20 °C\u003c\/dd\u003e\n\u003cdt\u003eStorage Comment\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e\n\u003col\u003e\n\u003cli\u003eFor unopened kit: All reagents should be stored according to the labels on the vials, so they are stable up to 6 months after receipt of the kit. The Reference Standard, Biotinylated Detection Antibody, HRP Conjugate and the 96-well stripe plate should be stored at -20 °C upon receipt while the other reagents should be stored at 4 °C.\u003c\/li\u003e\n\u003cli\u003eFor used kit: When the kit is used, the remaining reagents need to be stored according to the above storage condition. Besides, please return the unused wells to the foil pouch containing the desiccant pack, and zip-seal the foil pouch.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003c\/dd\u003e\n\u003cdt\u003eExpiry Date\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003e6 months\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003c\/li\u003e\n\u003cli id=\"section-target_details\" class=\"list-group-item list-group-item-action px-2 px-sm-0\"\u003e\n\u003cdiv class=\"mb-0\"\u003e\n\u003ch2 class=\"h4 fs-4 d-inline-block collapse-trigger\"\u003eTarget Details for COL2\u003c\/h2\u003e\n\u003c\/div\u003e\n\u003cdl id=\"productDetailstarget_details\" class=\"collapse show\"\u003e\n\u003cdt\u003eTarget\u003c\/dt\u003e\n\u003cdd\u003eCOL2 (Collagen, Type II (COL2))\u003c\/dd\u003e\n\u003cdt\u003eAlternative Name\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eCollagen Type II \u003c\/dd\u003e\n\u003cdt\u003eSynonyms\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003ePT ELISA Kit, RPRGL2 ELISA Kit, THPH1 ELISA Kit, Cf-2 ELISA Kit, Cf2 ELISA Kit, FII ELISA Kit, thrombin ELISA Kit, prothrombin ELISA Kit, CAP18 ELISA Kit, Cnlp ELISA Kit, Cramp ELISA Kit, FALL39 ELISA Kit, MCLP ELISA Kit, coagulation factor II, thrombin ELISA Kit, coagulation factor II ELISA Kit, cathelicidin antimicrobial peptide ELISA Kit, Cuticle collagen 2 ELISA Kit, collagen type II alpha 1 chain ELISA Kit, F2 ELISA Kit, Camp ELISA Kit, col-2 ELISA Kit, COL2A1 ELISA Kit\u003c\/dd\u003e\n\u003cdt\u003eBackground\u003c\/dt\u003e\n\u003cdd class=\"text-break mb-3\"\u003eCOL2\u003c\/dd\u003e\n\u003c\/dl\u003e\n\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"ANTIBODIES-ONLINE","offers":[{"title":"Default Title","offer_id":44895260803296,"sku":"ABIN6963607","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/unnamed_a0eaa1ee-e55f-4ccb-bec8-fbbfae10a7f9.jpg?v=1709539107","url":"https:\/\/rndmate.com\/products\/col2-elisa-kit","provider":"알앤디메이트","version":"1.0","type":"link"}