{"product_id":"cusabio-csb-ma000011m1m-100ul","title":"His Monoclonal Antibody","description":"\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRelease Date\u003c\/th\u003e\n\u003ctd\u003e2023-12-13\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Code\u003c\/th\u003e\n\u003ctd\u003eCSB-MA000011M1m\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShort Name\u003c\/th\u003e\n\u003ctd\u003eHis Monoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003eMonoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eList Price\u003c\/th\u003e\n\u003ctd\u003e$200\/100ul, $120\/50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eSpecies Independent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, WB, IP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eWB:1:1000-1:640000, IP:2µl-5µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eProtein G purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Formulation\u003c\/th\u003e\n\u003ctd\u003e0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eThe immunogen is a mixture of various His-tagged proteins\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen Species\u003c\/th\u003e\n\u003ctd\u003eSpecies Independent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Name\u003c\/th\u003e\n\u003ctd\u003eHis Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClone ID\u003c\/th\u003e\n\u003ctd\u003e2F4B5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003ctd\u003eNon-conjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Condition\u003c\/th\u003e\n\u003ctd\u003eUpon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTDS URL\u003c\/th\u003e\n\u003ctd\u003ehttps:\/\/www.cusabio.com\/datasheet\/12935188\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e100ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003ch3\u003eImage 1\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in: 200ng recombinant protein with His tag\u003cbr\u003eHis antibody at 1:8w, 1:16w, 1:32w, 1:64w, 1:128w, 1:256w\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 43.4 KDa\u003cbr\u003e\u003cbr\u003eObserved band size: 43.4 KDa\u003cbr\u003e\u003cbr\u003eExposure time：5min\u003c\/p\u003e\u003ch3\u003eImage 2\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in: 200ng recombinant protein with His tag\u003cbr\u003eHis antibody at 1:2w, 1:4w, 1:8w, 1:16w, 1:32w, 1:64w\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size:62.5 KDa\u003cbr\u003e\u003cbr\u003eObserved band size: 66 KDa\u003cbr\u003e\u003cbr\u003eExposure time：5min\u003c\/p\u003e\u003ch3\u003eImage 3\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in:200ng of four Recombinant protein with his tag\u003cbr\u003eAll lanes: His antibody at 1:5000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 43.4, 62.5,56,80.6 KDa\u003cbr\u003e\u003cbr\u003eObserved band size:43.4, 66, 57 ,90 KDa\u003cbr\u003e\u003cbr\u003eExposure time：5min\u003c\/p\u003e\u003ch3\u003eImage 4\u003c\/h3\u003e\u003cp\u003eImmunoprecipitating His in 293 whole cell lysate transfected with His \u003cbr\u003e\u003cbr\u003eLane 1: control IgG instead of 2F4B5 in 293 whole cell lysate transfected with His.\u003cbr\u003eLane 2: 2F4B5 antibody(2µg) + 293 whole cell lysate transfected with His (500µg) \u003cbr\u003e\u003cbr\u003eLane 3: 293 whole cell lysate transfected with His (5µg) \u003cbr\u003e\u003cbr\u003eFor western blotting, the blot was detected with 2F4B5 antibody at 1:10000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000 \u003cbr\u003e\u003c\/p\u003e","brand":"Cusabio","offers":[{"title":"100ul","offer_id":68540414427360,"sku":"CSB-MA000011M1m","price":200.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/CSB-MA000011M1m-WB-1.jpg?v=1794059150","url":"https:\/\/rndmate.com\/products\/cusabio-csb-ma000011m1m-100ul","provider":"알앤디메이트","version":"1.0","type":"link"}