{"product_id":"cusabio-csb-ma000091m1m-50ul","title":"ACTB Monoclonal Antibody","description":"\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRelease Date\u003c\/th\u003e\n\u003ctd\u003e2022-04-01\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Code\u003c\/th\u003e\n\u003ctd\u003eCSB-MA000091M1m\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShort Name\u003c\/th\u003e\n\u003ctd\u003eACTB Monoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003eMonoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eList Price\u003c\/th\u003e\n\u003ctd\u003e$200\/100ul, $120\/50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Mouse, Rat, Rabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, WB, IHC, IF, FC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eWB:1:5000-1:80000, IHC:1:500-1:1000, IF:1:50-1:200, FC:1:100-1:300\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eProtein A purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Formulation\u003c\/th\u003e\n\u003ctd\u003e0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eA synthesized peptide derived from human Beta-Actin (1-50aa)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen Species\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniprot ID\u003c\/th\u003e\n\u003ctd\u003eP60709\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Name\u003c\/th\u003e\n\u003ctd\u003eACTB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClone ID\u003c\/th\u003e\n\u003ctd\u003e1D12E12B5\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG2b\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003ctd\u003eNon-conjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Condition\u003c\/th\u003e\n\u003ctd\u003eUpon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTDS URL\u003c\/th\u003e\n\u003ctd\u003ehttps:\/\/www.cusabio.com\/datasheet\/12930731\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003ch3\u003eImage 1\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e Positive WB detected in: 293T whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, NIH\/3T3 whole cell lysate, K562 whole cell lysate, Rat spleen tissue, Rabbit kidney tissue\u003cbr\u003e All lanes: ACTB antibody at 1:5000\u003cbr\u003e Secondary\u003cbr\u003e Goat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e Predicted band size: 42 KDa\u003cbr\u003e Observed band size: 42 KDa\u003cbr\u003e Exposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 2\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, Hela whole cell lysate, NIH\/3T3 whole cell lysate, Mouse kidney tissue, Rabbit kidney tissue\u003cbr\u003e All lanes: ACTB antibody at 1:5000\u003cbr\u003e Secondary\u003cbr\u003e Goat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e Predicted band size: 42 KDa\u003cbr\u003e Observed band size: 42 KDa\u003cbr\u003e Exposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 3\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e Positive WB detected in: Mouse liver tissue, Mouse brain tissue, Mouse lung tissue, Mouse kidney tissue, Mouse spleen tissue, Mouse stomach tissue \u003cbr\u003e All lanes: ACTB antibody at 1:5000\u003cbr\u003e Secondary\u003cbr\u003e Goat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e Predicted band size: 42 KDa\u003cbr\u003e Observed band size: 42 KDa\u003cbr\u003e Exposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 4\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e Positive WB detected in: Rabbit liver tissue, Rabbit brain tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit stomach tissue, A549 whole cell lysate\u003cbr\u003e All lanes: ACTB antibody at 1:5000\u003cbr\u003e Secondary\u003cbr\u003e Goat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e Predicted band size: 42 KDa\u003cbr\u003e Observed band size: 42 KDa\u003cbr\u003e Exposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 5\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e Positive WB detected in: Rat liver tissue, Rat brain tissue, Rat lung tissue, Rat stomach tissue, U87 whole cell lysate \u003cbr\u003e All lanes: ACTB antibody at 1:5000\u003cbr\u003e Secondary\u003cbr\u003e Goat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e Predicted band size: 42 KDa\u003cbr\u003e Observed band size: 42 KDa\u003cbr\u003e Exposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 6\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e Positive WB detected in: 293T whole cell lysate at 40μg, 20μg, 10μg\u003cbr\u003e All lanes:ACTB antibody at 1:5000\u003cbr\u003e Secondary\u003cbr\u003e Goat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e Predicted band size: 42 KDa\u003cbr\u003e Observed band size: 42 KDa\u003cbr\u003e Exposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 7\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e Positive WB detected in: 20μg 293T whole cell lysate\u003cbr\u003e ACTB antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000\u003cbr\u003e Secondary\u003cbr\u003e Goat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e Predicted band size: 42 KDa\u003cbr\u003e Observed band size: 42 KDa\u003cbr\u003e Exposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 8\u003c\/h3\u003e\u003cp\u003eIHC image of CSB-MA000091M1m diluted at 1:500 and staining in paraffin-embedded colon  cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37鈩?Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.\u003c\/p\u003e\u003ch3\u003eImage 9\u003c\/h3\u003e\u003cp\u003eIHC image of CSB-MA000091M1m diluted at 1:500 and staining in paraffin-embedded breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at 37鈩?Then primary antibody (1% BSA) was incubated at 4掳C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.\u003c\/p\u003e\u003ch3\u003eImage 10\u003c\/h3\u003e\u003cp\u003eImmunofluorescence staining of Hela cells with CSB-MA000091M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG 锛圚+L锛?\u003c\/p\u003e\u003ch3\u003eImage 11\u003c\/h3\u003e\u003cp\u003eImmunofluorescence staining of HepG2 cells with CSB-MA000091M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4掳C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Mouse IgG 锛圚+L锛?\u003c\/p\u003e\u003ch3\u003eImage 12\u003c\/h3\u003e\u003cp\u003eOverlay Peak curve showing Hela cells stained with CSB-MA000091M1m (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1碌g\/1*106cells) for 1 h at 4掳C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1\/100 dilution for 30min at 4掳C. Isotype control antibody (green line) was mouse IgG2b (1碌g\/1*106cells) used under the same conditions. Acquisition of \u0026gt;10,000 events was performed.\u003c\/p\u003e\u003ch3\u003eImage 13\u003c\/h3\u003e\u003cp\u003eOverlay Peak curve showing HepG2 cells stained with CSB-MA000091M1m (red line) at 1:200. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1碌g\/1*106cells) for 1 h at 4掳C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1\/100 dilution for 30min at 4掳C. Isotype control antibody (green line) was mouse IgG2b (1碌g\/1*106cells) used under the same conditions. Acquisition of \u0026gt;10,000 events was performed.\u003c\/p\u003e","brand":"Cusabio","offers":[{"title":"50ul","offer_id":68333666795744,"sku":"CSB-MA000091M1m","price":120.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/CSB-MA000091M1m-WB-1_7a517bd2-45d7-43f7-b9fa-530ab6b0ae09.jpg?v=1793342032","url":"https:\/\/rndmate.com\/products\/cusabio-csb-ma000091m1m-50ul","provider":"알앤디메이트","version":"1.0","type":"link"}