{"product_id":"cusabio-csb-ma000161m1m-50ul","title":"V5-Tag Monoclonal Antibody","description":"\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRelease Date\u003c\/th\u003e\n\u003ctd\u003e2022-04-01\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Code\u003c\/th\u003e\n\u003ctd\u003eCSB-MA000161M1m\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShort Name\u003c\/th\u003e\n\u003ctd\u003eV5-Tag Monoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003eMonoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eList Price\u003c\/th\u003e\n\u003ctd\u003e$200\/100ul, $120\/50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eSpecies Independent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, WB, IF, IP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eWB:1:5000-1:1280000, IF:1:100-1:200, IP:2µl-5µl\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eProtein A purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Formulation\u003c\/th\u003e\n\u003ctd\u003e0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eGKPIPNPLLGLDST synthetic peptide conjugate to KLH\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen Species\u003c\/th\u003e\n\u003ctd\u003eSpecies Independent\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Name\u003c\/th\u003e\n\u003ctd\u003eV5-Tag\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClone ID\u003c\/th\u003e\n\u003ctd\u003e9E8B7\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG2a\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003ctd\u003eNon-conjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Condition\u003c\/th\u003e\n\u003ctd\u003eUpon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTDS URL\u003c\/th\u003e\n\u003ctd\u003ehttps:\/\/www.cusabio.com\/datasheet\/12931895\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003ch3\u003eImage 1\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in: V5-tagged fusion protein 1, 2, 3, 4, 5, 6 at 50ng\u003cbr\u003e\u003cbr\u003eAll lanes: V5 antibody at 1:2000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 31.9, 20.4, 55, 24.2, 53.4, 30 KDa\u003cbr\u003e\u003cbr\u003eObserved band size: 32, 21, 55, 28, 58, 33 KDa\u003cbr\u003e\u003cbr\u003eExposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 2\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in: 50ng V5-tagged fusion protein\u003cbr\u003e\u003cbr\u003eV5 Tag antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000, 1:1280000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to mouse IgG at 1\/50000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 32 KDa\u003cbr\u003e\u003cbr\u003eObserved band size: 32 KDa\u003cbr\u003e\u003cbr\u003eExposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 3\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in: Mouse anti V5 tag Monoclonal antibody at 1:2000\u003cbr\u003e\u003cbr\u003eLane 1: Recombinant V5-tagged fusion protein at 25ng\u003cbr\u003e\u003cbr\u003eLane 2: Recombinant V5-tagged fusion protein at 12.5ng\u003cbr\u003e\u003cbr\u003eLane 3: Recombinant V5-tagged fusion protein at 6.25ng\u003cbr\u003e\u003cbr\u003eLane 4: Recombinant V5-tagged fusion protein at 3.125ng\u003cbr\u003e\u003cbr\u003eLane 5: Recombinant V5-tagged fusion protein at 1.56ng\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/50000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 32 kd\u003cbr\u003e\u003cbr\u003eObserved band size: 32 kd\u003cbr\u003e\u003cbr\u003eExposure time: 5min\u003c\/p\u003e\u003ch3\u003eImage 4\u003c\/h3\u003e\u003cp\u003eImmunofluorescence staining of transfected HEK293F cells with CSB-MA000161M1m at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor R-PE Goat Anti-Mouse IgG(H+L).\u003c\/p\u003e\u003ch3\u003eImage 5\u003c\/h3\u003e\u003cp\u003eImmunoprecipitating V5 Tag in Transfected HEK293F cells whole cell lysate\u003cbr\u003e\u003cbr\u003eLane 1: Mouse control IgG2a instead of CSB-MA000161M1m in Transfected HEK293 cells whole cell lysate\u003cbr\u003e\u003cbr\u003eLane 2: CSB-MA000161M1m (2.5µl) + Transfected HEK293F cells whole cell lysate (500µg)\u003cbr\u003e\u003cbr\u003eLane 3: Transfected HEK293F cells whole cell lysate (20µg)\u003cbr\u003e\u003cbr\u003eFor western blotting, the blot was detected with CSB-MA000161M1m at 1:3000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000\u003c\/p\u003e","brand":"Cusabio","offers":[{"title":"50ul","offer_id":68539990376672,"sku":"CSB-MA000161M1m","price":120.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/CSB-MA000161M1m-WB-1_639acff9-ed21-4171-9a06-01f63705a3a3.jpg?v=1794057178","url":"https:\/\/rndmate.com\/products\/cusabio-csb-ma000161m1m-50ul","provider":"알앤디메이트","version":"1.0","type":"link"}