{"product_id":"cusabio-csb-ma754656a0m-50ul","title":"TUBA1A Monoclonal Antibody","description":"\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRelease Date\u003c\/th\u003e\n\u003ctd\u003e2022-04-01\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Code\u003c\/th\u003e\n\u003ctd\u003eCSB-MA754656A0m\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShort Name\u003c\/th\u003e\n\u003ctd\u003eTUBA1A Monoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003eMonoclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eList Price\u003c\/th\u003e\n\u003ctd\u003e$200\/100ul, $120\/50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Rabbit, Rat, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, WB, IHC, IF, FC, IP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eWB:1:20000-1:320000, IHC:1:100-1:300, IF:1:50-1:200, FC:1:100-1:300, IP:1µg-5µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eProtein A purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Formulation\u003c\/th\u003e\n\u003ctd\u003e0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003eA synthesized peptide derived from human Tubulin alpha-1A chain (297-309)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen Species\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Name\u003c\/th\u003e\n\u003ctd\u003eTUBA1A\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003eMonoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClone ID\u003c\/th\u003e\n\u003ctd\u003e7E5C12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eMouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG2a\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003ctd\u003eNon-conjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Condition\u003c\/th\u003e\n\u003ctd\u003eUpon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTDS URL\u003c\/th\u003e\n\u003ctd\u003ehttps:\/\/www.cusabio.com\/datasheet\/12927525\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003ch3\u003eImage 1\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in:  U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate, \u003cbr\u003e\u003cbr\u003eAll lanes: TUBA1A antibody at 1:5000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 2\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in:  Rabbit heatrt tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue\u003cbr\u003e\u003cbr\u003eAll lanes: TUBA1A antibody at 1:5000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 3\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in:  Rat brain tissue, Rat stomach tissue, Rat kidney tissue\u003cbr\u003e\u003cbr\u003eAll lanes: TUBA1A antibody at 1:5000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 4\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in:  NIH\/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissue\u003cbr\u003e\u003cbr\u003eAll lanes: TUBA1A antibody at 1:5000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 5\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in:  Hela whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg\u003cbr\u003e\u003cbr\u003eAll lanes: TUBA1A antibody at 1:5000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 6\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in: Hela whole cell lysate\u003cbr\u003e\u003cbr\u003eAll lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 7\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in:  Hela whole cell lysate at 20µg, 10µg, 5µg, 2.5µg, 1.25µg, 0.625µg, 0.3125µg, 0.15625µg\u003cbr\u003e\u003cbr\u003eAll lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:5000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 8\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003ePositive WB detected in: Hela whole cell lysate\u003cbr\u003e\u003cbr\u003eAll lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to Mouse IgG at 1\/10000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 52 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 52 kDa\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 9\u003c\/h3\u003e\u003cp\u003eIHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.\u003c\/p\u003e\u003ch3\u003eImage 10\u003c\/h3\u003e\u003cp\u003eIHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.\u003c\/p\u003e\u003ch3\u003eImage 11\u003c\/h3\u003e\u003cp\u003eImmunofluorescence staining of Hela cells with CSB-MA754656A0m at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).\u003c\/p\u003e\u003ch3\u003eImage 12\u003c\/h3\u003e\u003cp\u003eImmunoprecipitating TUBA1A in Hela whole cell lysate\u003cbr\u003e\u003cbr\u003eLane 1: Mouse control IgG (1µg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1\/2000)\u003cbr\u003e\u003cbr\u003eLane 2: CSB-MA754656A0m (5µg) + Hela whole cell lysate (500µg)\u003cbr\u003e\u003cbr\u003eLane 3: Hela whole cell lysate (20µg)\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 13\u003c\/h3\u003e\u003cp\u003eImmunoprecipitating TUBA1A in Hela whole cell lysate\u003cbr\u003e\u003cbr\u003eLane 1: Mouse control IgG (1µg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1\/2000)\u003cbr\u003e\u003cbr\u003eLane 2: Company A (5µg), Company B (5µg), Company C (5µg), Company D (5µg), CSB-MA754656A0m (5µg) + Hela whole cell lysate (500µg)\u003cbr\u003e\u003cbr\u003eLane 3: Hela whole cell lysate (20µg)\u003cbr\u003e\u003c\/p\u003e\u003ch3\u003eImage 14\u003c\/h3\u003e\u003cp\u003eOverlay histogram showing Hela cells stained with CSB-MA754656A0m (red line) at 1:150. The cells were incubated in 1x PBS \/10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1\/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of \u0026gt;10,000 events was performed.\u003c\/p\u003e\u003ch3\u003eImage 15\u003c\/h3\u003e\u003cp\u003eOverlay histogram showing Hela cells stained with Company A (5µg), Company B (5µg), Company C (5µg), Company D (5µg), CSB-MA754656A0m (5µg) (red line) at 1:150. The cells were incubated in 1x PBS \/10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1\/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of \u0026gt;10,000 events was performed.\u003c\/p\u003e","brand":"Cusabio","offers":[{"title":"50ul","offer_id":68333665386720,"sku":"CSB-MA754656A0m","price":120.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/CSB-MA754656A0m-WB-1_389bf830-a485-4d14-bd19-1d446e92bd47.jpg?v=1793342009","url":"https:\/\/rndmate.com\/products\/cusabio-csb-ma754656a0m-50ul","provider":"알앤디메이트","version":"1.0","type":"link"}