{"product_id":"cusabio-csb-pa010378pa96achu-50ul","title":"Acetyl-HIST1H1C (K96) Antibody","description":"\u003ctable\u003e\n\u003ctr\u003e\n\u003cth\u003eRelease Date\u003c\/th\u003e\n\u003ctd\u003e2022-04-01\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct Code\u003c\/th\u003e\n\u003ctd\u003eCSB-PA010378PA96acHU\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eShort Name\u003c\/th\u003e\n\u003ctd\u003eAcetyl-HIST1H1C (K96) Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntibody Type\u003c\/th\u003e\n\u003ctd\u003ePolyclonal Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eList Price\u003c\/th\u003e\n\u003ctd\u003e$319\/100ul, $175\/50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTested Applications\u003c\/th\u003e\n\u003ctd\u003eELISA, WB, ICC, IF, IP, ChIP\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDilution\u003c\/th\u003e\n\u003ctd\u003eWB:1:1000-1:5000, ICC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurification Method\u003c\/th\u003e\n\u003ctd\u003eAntigen affinity purification\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eBuffer Formulation\u003c\/th\u003e\n\u003ctd\u003e0.03% Proclin 300, 50% Glycerol, 0.01M PBS, pH 7.4.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen\u003c\/th\u003e\n\u003ctd\u003ePeptide sequence around site of Acetyl-Lys (96) derived from human Histone H1.2\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eAntigen Species\u003c\/th\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eUniprot ID\u003c\/th\u003e\n\u003ctd\u003eP16403\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTarget Name\u003c\/th\u003e\n\u003ctd\u003eHIST1H1C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eClonality\u003c\/th\u003e\n\u003ctd\u003ePolyclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eHost Species\u003c\/th\u003e\n\u003ctd\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eIsotype\u003c\/th\u003e\n\u003ctd\u003eIgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eConjugation\u003c\/th\u003e\n\u003ctd\u003eNon-conjugated\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eForm\u003c\/th\u003e\n\u003ctd\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage Condition\u003c\/th\u003e\n\u003ctd\u003eUpon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTDS URL\u003c\/th\u003e\n\u003ctd\u003ehttps:\/\/www.cusabio.com\/datasheet\/12782899\/\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSize\u003c\/th\u003e\n\u003ctd\u003e50ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\u003ch3\u003eImage 1\u003c\/h3\u003e\u003cp\u003eWestern Blot\u003cbr\u003e\u003cbr\u003eDetected samples: Hela whole cell lysate, 293 whole cell lysate, K562 whole cell lysate, Jurkat whole cell lysate; Untreated (-) or treated (+) with 30mM sodium butyrate for 4h\u003cbr\u003e\u003cbr\u003eAll lanes: HIST1H1C antibody at 1:2000\u003cbr\u003e\u003cbr\u003eSecondary\u003cbr\u003e\u003cbr\u003eGoat polyclonal to rabbit IgG at 1\/40000 dilution\u003cbr\u003e\u003cbr\u003ePredicted band size: 22 kDa\u003cbr\u003e\u003cbr\u003eObserved band size: 22 kDa\u003c\/p\u003e\u003ch3\u003eImage 2\u003c\/h3\u003e\u003cp\u003eImmunocytochemistry analysis of Hela cells using CSB-PA010378PA96acHU at dilution of 1:100\u003c\/p\u003e\u003ch3\u003eImage 3\u003c\/h3\u003e\u003cp\u003eImmunofluorescent analysis of Hela cells (sodium butyrate, 30 mM, 4h) using CSB-PA010378PA96acHU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)\u003c\/p\u003e\u003ch3\u003eImage 4\u003c\/h3\u003e\u003cp\u003eChromatin Immunoprecipitation Hela (4*10\u0026lt;sup\u0026gt;6\u0026lt;\/sup\u0026gt;, treated with 30mM sodium butyrate for 4h) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 8µg anti-HIST1H1C (CSB-PA010378PA96acHU) or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers against the β-Globin promoter.\u003c\/p\u003e\u003ch3\u003eImage 5\u003c\/h3\u003e\u003cp\u003eImmunoprecipitating HIST1H1C in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h)\u003cbr\u003e\u003cbr\u003eLane 1: Rabbit control IgG instead of CSB-PA010378PA96acHU in Hela whole cell lysate (treated with 30mM sodium butyrate for 4h). For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1\/2000)\u003cbr\u003e\u003cbr\u003eLane 2: CSB-PA010378PA96acHU (5µg) + Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (500µg)\u003cbr\u003e\u003cbr\u003eLane 3: Hela whole cell lysate (treated with 30mM sodium butyrate for 4h) (20µg)\u003c\/p\u003e","brand":"Cusabio","offers":[{"title":"50ul","offer_id":68510381441248,"sku":"CSB-PA010378PA96acHU","price":175.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/CSB-PA010378PA96acHU-WB_260f065d-1509-4007-b67b-dcba698a2969.jpg?v=1793962992","url":"https:\/\/rndmate.com\/products\/cusabio-csb-pa010378pa96achu-50ul","provider":"알앤디메이트","version":"1.0","type":"link"}