{"product_id":"elk10386","title":"Human F8(Coagulation Factor VIII) ELISA Kit","description":"\u003cstrong\u003eAlternative Names:\u003c\/strong\u003e FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein\u003cbr\u003e\u003cstrong\u003eAssay Type:\u003c\/strong\u003e Sandwich\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e 0.12 IU\/mL\u003cbr\u003e\u003cstrong\u003estandard:\u003c\/strong\u003e 20 IU\/mL\u003cbr\u003e\u003cstrong\u003eDetection range:\u003c\/strong\u003e 0.32-20 IU\/mL\u003cbr\u003e\u003cstrong\u003eSample type:\u003c\/strong\u003e plasma\u003cbr\u003e\u003cstrong\u003eAssay length:\u003c\/strong\u003e 3.5h\u003cbr\u003e\u003cstrong\u003eResearch Area:\u003c\/strong\u003e Metabolic pathway;Hematology;\u003cbr\u003e\u003cstrong\u003eTest principle:\u003c\/strong\u003e The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human F8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human F8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human F8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human F8 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003cbr\u003e\u003cstrong\u003eUniProt ID:\u003c\/strong\u003e P00451","brand":"ELK Biotechnology","offers":[{"title":"96T","offer_id":47539538886880,"sku":"ELK10386","price":558.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/ELK_6cde47e2-88eb-4f82-853b-e68810382486.jpg?v=1750999514","url":"https:\/\/rndmate.com\/products\/elk10386","provider":"알앤디메이트","version":"1.0","type":"link"}