{"product_id":"enzychrom™-catalase-assay-kit","title":"EnzyChrom™ Catalase Assay Kit","description":"\u003ch3 data-mce-fragment=\"1\"\u003eApplication\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eFor quantitative determination of catalase activity and evaluation of drug effects on catalase activity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eKey Features\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSensitive and accurate. Use 10 µL sample. Linear detection range 0.2 to 5 U\/L catalase activity.\u003c\/li\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSimple and Convenient. The procedure involves adding a Substrate to the sample, incubation for 30 min, followed by a Detection Reagent and reading the optical density or fluorescence intensity.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eMethod\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eOD570nm, or FL530\/585nm\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eSamples\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eSerum, plasma, urine, saliva, cell culture etc\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eSpecies\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003eAll\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eProcedure\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e40 min\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eSize\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e100 tests\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eDetection Limit\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e0.2 U\/L\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eShelf Life\u003c\/h3\u003e\n\u003cul data-mce-fragment=\"1\"\u003e\n\u003cli data-mce-fragment=\"1\"\u003e6 months\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3 data-mce-fragment=\"1\"\u003eMore Details\u003c\/h3\u003e\n\u003cp\u003e\u003ci data-mce-fragment=\"1\"\u003eCATALASE\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003c\/i\u003e\u003cspan data-mce-fragment=\"1\"\u003e(EC 1.11.1.6), is an ubiquitous antioxidant enzyme that catalyzes the decomposition of hydrogen peroxide (H\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003eO\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003e) to water and oxygen. By preventing excessive H\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003eO\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003e build-up, catalase allows important cellular processes which produce H\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003eO\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003e as a byproduct to occur safely. Deficiency in catalase activity has been associated with grey hair and peroxisomal disorder acatalasia. Simple, direct and high-throughput assays for catalase activity find wide applications. BioAssay Systems improved assay directly measures catalase degradation of H\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003eO\u003c\/span\u003e\u003csub data-mce-fragment=\"1\"\u003e2\u003c\/sub\u003e\u003cspan data-mce-fragment=\"1\"\u003e using a redox dye. The change in colour intensity at 570nm or fluorescence intensity (λex\/em = 530\/585nm) is directly proportional to the catalase activity in the sample.\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch3\u003eRegarding the technique used in the DPOD-100 peroxidase assay and the ECAT-100 catalase assay that seems to be really similar, how will we be sure that one of them will measure the catalase activity and the other the peroxidase?\u003c\/h3\u003e\n\u003cp\u003eThe DPOD assay measures the conversion of the dye reagent and hydrogen peroxide, which are provided in large excess as substrates for cellular peroxidases. Endogenous catalase activity does not interfere with the assay, because it does not react with the dye reagent, hydrogen peroxide is in large excess, and the reaction is short (10 minutes), leaving the H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003e \u003c\/span\u003econcentration practically unchanged.\u003c\/p\u003e\n\u003cp\u003eThe ECAT assays measures catalase activity by first incubating much lower concentrations of hydrogen peroxide with the samples for 30 minutes. Then the decrease in hydrogen peroxide is measured by adding the dye reagent and HRP in excess.\u003cbr class=\"\"\u003e\u003cbr class=\"\"\u003e\u003c\/p\u003e\n\u003ch3\u003eYou will have 100 µL of each standard when you have pipetted all solutions for the standard curve. How stable are the 100 µl standards? Can they be used the next day to make another standard curve or can the values from the already made standard curve be used when you have other samples the next day?\u003c\/h3\u003e\n\u003cp\u003eThe H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e\u003cspan\u003e \u003c\/span\u003estandards are stable for an hour. Therefore assays should be run as soon as possible with the samples and standards. It is recommended that customers run standard curve in each experiment.\u003c\/p\u003e","brand":"BioAssay Systems","offers":[{"title":"Default Title","offer_id":44598619439328,"sku":"ECAT-100","price":349.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/Screenshot2024-01-12103914.png?v=1705018331","url":"https:\/\/rndmate.com\/products\/enzychrom%e2%84%a2-catalase-assay-kit","provider":"알앤디메이트","version":"1.0","type":"link"}