{"product_id":"filgrastim-gcsf-pharmacokinetic-elisa-el-1611-73196-el-1611-73196","title":"Filgrastim (GCSF) Pharmacokinetic ELISA | EL-1611-73196","description":"\u003ch2\u003eFilgrastim (GCSF) Pharmacokinetic ELISA | EL-1611-73196\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffinityImmuno Filgrastim (GCSF) Pharmacokinetic ELISA | EL-1611-73196\u003c\/strong\u003e is a ready-to-use ELISA kit designed for pharmacokinetic (PK), anti-drug antibody (ADA), and immunogenicity workflows in biologics and biosimilar development.\u003c\/p\u003e\n\u003cp\u003eGranulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream\u003c\/p\u003e\n\u003ch3\u003eProduct Highlights\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget Drug:\u003c\/strong\u003e granulocyte colony stimulating factor (G-CSF)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct Type:\u003c\/strong\u003e ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplication:\u003c\/strong\u003e granulocyte colony stimulating factor in biological matrices\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMethod:\u003c\/strong\u003e Direct sandwich ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Method:\u003c\/strong\u003e Peroxidase \/ OD450\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Range:\u003c\/strong\u003e 9.375ng\/mL - 500 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Minimum:\u003c\/strong\u003e 9.375 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Type:\u003c\/strong\u003e Serum Plasma\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Volume:\u003c\/strong\u003e 15ul\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Time:\u003c\/strong\u003e 2.5 hours\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e -20C, 1 year\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e dry ice, less than -20C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePack Size:\u003c\/strong\u003e 1X96 wells\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e GCSF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlate Format:\u003c\/strong\u003e Strip\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Details\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eComponents:\u003c\/strong\u003e Coated microtiter plate, 96 wells (1x8 strips) 1 Calibrator diluent 1.8ml Calibrator (1mg\/ml) 12μl 20X wash buffer 25ml Assay buffer 50ml 1000X secondary antibody 1000X detection reagent 17μl TMB 12ml TMB stop solution 12ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Procedure:\u003c\/strong\u003e This assay employs the indirect sandwich enzyme immunoassay technique. Anti-GCSF is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. GCSF present in biological matrices is bound by the immobilized anti- GCSF antibody. After washing away any unbound substances, tagged anti-GCSF secondary antibody is added to the wells. After washing, diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of GCSF present in test samples. The color development is stopped and the intensity of the color is measured.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReagent Preparation:\u003c\/strong\u003e Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation: Dilute wash buffer\nconcentrate with ultra-pure water 1\/20 before use\n(for example add 25mL concentrate to 475mL ultrapure water). Mix well.\n2. Secondary Antibody (1X) Preparation: Dilute secondary\nantibody with assay buffer 1\/1000 before use (for\nexample add 12μl to 12mL of assay buffer). Mix well.\n3. Detection Reagent (1X) Preparation: Dilute detection\nreagent with assay buffer 1\/1000 before use (for\nexample add 12μl concentrate to 12ml of assay buffer).\nMix well.\n4. Calibrator Preparation: Dilute the calibrator from 1mg\/ml\ndown to 5µg\/ml by pipetting 5µL of calibrator stock into\n995µL assay buffer. Label \"Cal. Int.\" Mix well. Prepare\ncalibrators with concentrations ranging from 500 ng\/ml\nto 9.375 ng\/ml. The following is an example calibrator\ncurve.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResults Calculation:\u003c\/strong\u003e 1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. 2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample. 3. Any sample diluted more or less than the standard series will need additional data correction. For example, if the sample is diluted 1\/50, then the concentration will be calculated by dividing by 2 due to the calibrators being 2 times more diluted. Similarly, if the sample is diluted 1\/500, then the concentration will be calculated by multiplying by a correction factor of 5 due to the calibrators being 5 times more concentrated.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Collection:\u003c\/strong\u003e This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Preparation:\u003c\/strong\u003e Dilute calibrators and test samples 1\/50 with assay buffer (for example add 5µL of prepared calibrator or sample to 245µL of assay buffer). Mix well. Do not store diluted samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Precision:\u003c\/strong\u003e \u0026lt; 25% for ULOQ and LLOQ and \u0026lt;20% for the remaining concentrations\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePreservative:\u003c\/strong\u003e None\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGene ID:\u003c\/strong\u003e 1440\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eSEO Keywords:\u003c\/strong\u003e granulocyte colony stimulating factor (G-CSF), Filgrastim (GCSF) Pharmacokinetic ELISA | EL-1611-73196, AffinityImmuno, ELISA, PK assay, ADA assay, biologics analysis, immunogenicity, bioanalytical testing, pharmacokinetic ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDatasheet:\u003c\/strong\u003e \u003ca href=\"https:\/\/affinityimmuno.com\/product\/gcsf-pharmacokinetic-elisa-el-1611-73196\/\"\u003ehttps:\/\/affinityimmuno.com\/product\/gcsf-pharmacokinetic-elisa-el-1611-73196\/\u003c\/a\u003e\u003c\/p\u003e","brand":"Affinity Immuno","offers":[{"title":"Default Title","offer_id":50127972335840,"sku":"EL-1611-73196","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/AffinityImmuno-Product-3_67e6297e-7d2d-41cb-aeff-42f4c9691d87.jpg?v=1773910679","url":"https:\/\/rndmate.com\/products\/filgrastim-gcsf-pharmacokinetic-elisa-el-1611-73196-el-1611-73196","provider":"알앤디메이트","version":"1.0","type":"link"}