{"product_id":"gcsf-ada-granulocyte-colony-stimulating-factor-elisa-el-141-73196","title":"GCSF ADA (granulocyte colony stimulating factor) ELISA","description":"\u003ch2\u003eGCSF ADA (granulocyte colony stimulating factor) ELISA\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffinityImmuno GCSF ADA (granulocyte colony stimulating factor) ELISA\u003c\/strong\u003e is a ready-to-use ELISA kit designed for pharmacokinetic (PK), anti-drug antibody (ADA), and immunogenicity workflows in biologics and biosimilar development.\u003c\/p\u003e\n\u003cp\u003eGranulocyte colony-stimulating factor (G-CSF or GCSF), also known as colony-stimulating factor 3 (CSF 3), is a glycoprotein that stimulates the bone marrow to produce granulocytes and stem cells and release them into the bloodstream\u003c\/p\u003e\n\u003ch3\u003eProduct Highlights\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget Drug:\u003c\/strong\u003e antibodies to GCSF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct Type:\u003c\/strong\u003e ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplication:\u003c\/strong\u003e Quantification of antibodies to G-CSF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMethod:\u003c\/strong\u003e Direct sandwich ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Method:\u003c\/strong\u003e Peroxidase \/ OD450\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e human\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Range:\u003c\/strong\u003e 45 ng\/mL -1440 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Minimum:\u003c\/strong\u003e 45 ng\/mL\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Type:\u003c\/strong\u003e Serum Plasma\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Volume:\u003c\/strong\u003e 15ul\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Time:\u003c\/strong\u003e 2.5 hours\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e -20C, 1 year\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e dry ice, less than -20C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePack Size:\u003c\/strong\u003e 1X96 wells\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e anti-GCSF antibodies\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlate Format:\u003c\/strong\u003e Strip\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Details\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eComponents:\u003c\/strong\u003e Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg\/mL) 12 μl 10X wash buffer 50 ml Assay buffer 50 ml 1000X detection reagent 17 μl TMB 12 ml TMB stop solution\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Procedure:\u003c\/strong\u003e This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReagent Preparation:\u003c\/strong\u003e Prepare only the appropriate amount of required\nreagent on the day of use. Store all reagents as per\ninstructions stated on the label.1. Wash Buffer (1X) Preparation:Dilute wash buffer concentrate with ultra-pure water 1\/20 before use (for example, add 50mL wash buffer concentrate to 450mL ultra-pure water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1\/1000 before use (for example add 11μl concentrate to 11ml of assay buffer). Mix well. 3. Calibrator Preparation: Dilute the calibrator from 1.15mg\/ml down to 11.5µg\/ml by pipetting 5µl of calibrator stock into 495µl assay buffer. Label \"Cal. Int.\" Mix well. Prepare calibrators with concentrations ranging from 1440ng\/ml to 45ng\/ml. The following is an example calibrator curve\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResults Calculation:\u003c\/strong\u003e 1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. 2. The concentration of the unknowns can be back calculated directly from this standard curve using the absorbance value for each sample.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Collection:\u003c\/strong\u003e This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Preparation:\u003c\/strong\u003e Dilute calibrators and test samples 1\/50 with assay buffer (for example add 5µl of prepared calibrator or sample to 245µl of assay buffer). Mix well. Do not store diluted samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Precision:\u003c\/strong\u003e \u0026lt;20%, \u0026lt;20%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePreservative:\u003c\/strong\u003e none\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGene ID:\u003c\/strong\u003e N\/ap\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eSEO Keywords:\u003c\/strong\u003e antibodies to GCSF, GCSF ADA (granulocyte colony stimulating factor) ELISA, AffinityImmuno, ELISA, PK assay, ADA assay, biologics analysis, immunogenicity, bioanalytical testing, pharmacokinetic ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDatasheet:\u003c\/strong\u003e \u003ca href=\"https:\/\/affinityimmuno.com\/wp-content\/uploads\/2022\/11\/EL-141-73196-GCSF-ADA-PRODUCT-SHEET.pdf\"\u003ehttps:\/\/affinityimmuno.com\/wp-content\/uploads\/2022\/11\/EL-141-73196-GCSF-ADA-PRODUCT-SHEET.pdf\u003c\/a\u003e\u003c\/p\u003e","brand":"Affinity Immuno","offers":[{"title":"Default Title","offer_id":50127972729056,"sku":"EL-141-73196","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/AffinityImmuno-Product-3_cdef7a65-a6e1-4b22-b6c3-b82498f6eac3.jpg?v=1773910686","url":"https:\/\/rndmate.com\/products\/gcsf-ada-granulocyte-colony-stimulating-factor-elisa-el-141-73196","provider":"알앤디메이트","version":"1.0","type":"link"}