{"product_id":"glass-plating-beads-sterilized","title":"Glass Plating Beads (Sterilized)","description":"\u003ch1\u003eGlass Plating Beads (Sterilized)\u003c\/h1\u003e\n\u003cbr\u003e\u003cstrong\u003eCat. No.\u003c\/strong\u003e\u003cspan\u003e: FYO001-100G\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eDescription\u003c\/strong\u003e\u003cbr\u003e\u003cspan\u003eSterilized glass plating beads (4 mm) provide an efficient and easy method to achieve optimal spreading on agar plates. The rolling action of the glass beads gently spread the E. coli solution (or any bacterial \/fungal solution) to reach areas of the plate that are inaccessible to spread. Multiple plates containing beads and E. coli solution may be stacked and shaken simultaneously to increase spreading throughout and reduce cell stress. The beads may be recovered by washing with 70% ethanol, then sterilized and dried.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eFeatures\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003cul\u003e\n\u003cli\u003ePre-sterilized\u003c\/li\u003e\n\u003cli\u003eNo flaming required\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eApplications\u003c\/strong\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003cul\u003e\n\u003cli\u003eBacterial and fungal solution spreading on agar plates\u003c\/li\u003e\n\u003cli\u003ePlating yeast for two-hybrid screens\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cbr\u003e\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003e#  YBB- FYO001-100G :  Sterilized, 4 mm, 100 g\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e \u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eQuality Control\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003eEach lot of beads has to pass contamination test by spreading sterilized water on agar plates containing no selective antibiotics. No bacterial and fugal colonies should be seen after 2 day incubation.\u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStore\u003c\/strong\u003e\u003cspan\u003e at  room temperature \u003c\/span\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eProtocol\u003cbr\u003e\u003cimg src=\"http:\/\/diagnocine.com\/Content\/Upload\/BlogImages\/beads-protocol_Protocol_680x176_Diagnocine.jpg\" alt=\"\"\u003e\u003c\/strong\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e\u003cspan\u003eFor regular plating of ECOS™ Competent Cells, add 5 ~ 10 beads immediately after pipetting 50 ~ 100 μl of transformed cells onto each plate. Move the plate back and forth to roll the beads on the surface and change the direction of shaking a few times to ensure even spreading. When the surface of the agar starts to dry on the plate (20 ~ 40 seconds), pour off the beads and the plates are ready for incubation.\u003c\/span\u003e\u003cbr\u003e\u003cspan\u003e   \u003c\/span\u003e\n\u003cul\u003e\n\u003cli\u003ePre-warm glass beads at 37°C for ECOS™ warm plating (protocol 2\u0026amp;4, page3-3)\u003c\/li\u003e\n\u003cli\u003ePre-chill glass beads at 4°C for ECOS™ cold plating (protocol 1\u0026amp;3, page 3-3)\u003c\/li\u003e\n\u003cli\u003eRoom temperature for SCOS and YLOS Transformation Kits\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Yeastern Biotech","offers":[{"title":"Default Title","offer_id":44839956381920,"sku":"FYO001-100G","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/Glass-Plating-Beads_Sterilized_YB-FYO001-100G_600x600_Diagnocine_1200.jpg?v=1708737738","url":"https:\/\/rndmate.com\/products\/glass-plating-beads-sterilized","provider":"알앤디메이트","version":"1.0","type":"link"}