{"product_id":"hsa-mir-181a-5p-mimic","title":"hsa-miR-181a-5p mimic","description":"\u003cmeta charset=\"utf-8\"\u003e\n\u003ctable class=\"details_info_tbl\" data-mce-fragment=\"1\"\u003e\n\u003ctbody data-mce-fragment=\"1\"\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eDescription\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ehsa-miR-181a-5p mimics are small, chemically synthesized double-stranded RNAs that mimic endogenous miRNAs and enable miRNA functional analysis by up-regulation of miRNA activity.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"font_italic details_info_th\" data-mce-fragment=\"1\"\u003eIn Vitro\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e1. miRNA Resuspension\u003c\/b\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1.1 Briefly centrifuge the tube to ensure that the dried miRNA is at the bottom of the tube.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1.2 Resuspend the miRNA using nuclease free water to generate 20 μM stock solution.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor 5 nmol miRNA: add 250 μL nuclease free water.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor 20 nmol miRNA: add 1000 μL nuclease free water.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1.3 Aliquot miRNAs into one or more tubes to limit the number of freeze-thaw cycles (\u0026lt;5).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e1.4 Store at or below -20°C or -80°C in a non-frost-free freezer until use.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e2. Prepare cells\u003c\/b\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e2.1 Inoculate cells in advance for\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/kits\/cell-transfection.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/kits\/cell-transfection.html\" target=\"_blank\"\u003ecell transfection\u003c\/a\u003e. The viability and general health of cells prior to transfection significantly affect transfection result.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003e3. Transfection\u003c\/b\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3.1 Prepare transfection mix A and B.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor per well of a 6-well plate: A: 120 μL serum-free medium + 5 μL\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" target=\"_blank\"\u003emiRNA mimics\u003c\/a\u003e; B: 121 μL serum-free medium + 4 μL\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" target=\"_blank\"\u003ePolyFast Transfection Reagent\u003c\/a\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e(\u003ca href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" target=\"_blank\"\u003eHY-K1014\u003c\/a\u003e).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor per well of a 24-well plate: A: 23.75 μL serum-free medium + 1.25 μL\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" target=\"_blank\"\u003emiRNA mimics\u003c\/a\u003e; B: 24 μL serum-free medium + 1 μL\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" target=\"_blank\"\u003ePolyFast Transfection Reagent\u003c\/a\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e(\u003ca href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" target=\"_blank\"\u003eHY-K1014\u003c\/a\u003e).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor per well of a 96-well plate: A: 4.75 μL serum-free medium + 0.25 μL\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" target=\"_blank\"\u003emiRNA mimics\u003c\/a\u003e; B: 4.8 μL serum-free medium + 0.2 μL\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" target=\"_blank\"\u003ePolyFast Transfection Reagent\u003c\/a\u003e\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e(\u003ca href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/inhibitor-kit\/polyfast-transfection-reagent.html\" target=\"_blank\"\u003eHY-K1014\u003c\/a\u003e).\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eNote:\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003c\/b\u003eThe recommended working concentration is 50nM for\u003cspan data-mce-fragment=\"1\"\u003e \u003c\/span\u003e\u003ca href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.medchemexpress.com\/oligonucleotides\/mimics.html\" target=\"_blank\"\u003emiRNA mimics\u003c\/a\u003e. miRNA function can vary greatly, depending on the miRNA, the cell line, and the chosen analysis method. To determine the concentration that provides optimal results, optimization experiments using varying mimic\/inhibitor concentrations should be performed. The optimized range suggests changing the miRNA concentration in the range of 10 to 200nM.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eIf other transfection reagents are used, the amount of transfection reagent needs to be adjusted according to the specific situation.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3.2 Mix A and B gently. Incubate at room temperature for 15 minutes.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3.3 Remove culture medium from cells, wash with PBS.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3.4 Add transfection mix (A+B) to cells.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor per well of a 6-well plate: add 1750 μL fresh medium without Pen\/Strep, then add 250 μL of the transfection mix (A+B) to the well, and mix well.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor per well of a 24-well plate: add 450 μL fresh medium without Pen\/Strep, then add 50 μL of the transfection mix (A+B) to the well, and mix well.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003eFor per well of a 96-well plate: add 90 μL fresh medium without Pen\/Strep, then add 10 μL of the transfection mix (A+B) to the well, and mix well.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e3.5 Incubate cells for 1–3 days at 37°C. Then, analyze transfected cells. The medium can be replaced with fresh serum-containing medium after 6 hours if necessary.\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e \u003cbr data-mce-fragment=\"1\"\u003e\u003c\/p\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003cb data-mce-fragment=\"1\"\u003eMedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.\u003c\/b\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eMolecular Weight\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp id=\"molecularWeight\" data-mce-fragment=\"1\"\u003e14632.82\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003emiRBase Accession Number\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e\u003ca class=\"link-product\" href=\"https:\/\/www.mirbase.org\/cgi-bin\/mirna_entry.pl?acc=MI0000289\" rel=\"nofollow\" data-mce-fragment=\"1\" data-mce-href=\"https:\/\/www.mirbase.org\/cgi-bin\/mirna_entry.pl?acc=MI0000289\" target=\"_blank\"\u003eMI0000289\u003c\/a\u003e\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eMature miRNA Sequence\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eAACAUUCAACGCUGUCGGUGAGU\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eStem-loop ID\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ehsa-mir-181a-1\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eStem-loop Sequence\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eUGAGUUUUGAGGUUGCUUCAGUGAACAUUCAACGCUGUCGGUGAGUUUGGAAUUAAAAUCAAAACCAUCGACCGUUGAUUGUACCCUAUGGCUAACCAUCAUCUACUCCA\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eSpecies\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eHuman, Mouse, Rat, Alligator mississippiensis, Anolis carolinensis, Callithrix jacchus, Chicken, Chrysemys picta, Cricetulus griseus, Daubentonia madagascariensis, Fugu rubripes, Gadus morhua, Gorilla gorilla, Horse, Lagothrix lagotricha, Macaca nemestrina, Monodelphis domestica, Nomascus leucogenys, Ornithorhynchus anatinus, Otolemur garnettii, Pan paniscus, Pan troglodytes, Papio hamadryas, Petromyzon marinus, Pongo pygmaeus, Rhesus monkey, Saguinus labiatus, Salmo salar, Sheep, Taeniopygia guttata, Tetraodon nigroviridis, Tupaia chinensis, Xenopus tropicalis, Zebrafish\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eSMILES\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003e[hsa-miR-181a-5p mimic]\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eShipping\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003eRoom temperature in continental US; may vary elsewhere.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr data-mce-fragment=\"1\"\u003e\n\u003cth class=\"details_info_th\" data-mce-fragment=\"1\"\u003eStorage\u003c\/th\u003e\n\u003ctd class=\"details_info_td\" data-mce-fragment=\"1\"\u003e\n\u003cdiv id=\"details_storage\" data-mce-fragment=\"1\"\u003e\n\u003cp data-mce-fragment=\"1\"\u003ePlease store the product under the recommended conditions in the Certificate of Analysis.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"MedChemExpress","offers":[{"title":"Default Title","offer_id":44793645727968,"sku":"HY-R00329","price":0.0,"currency_code":"KRW","in_stock":true}],"url":"https:\/\/rndmate.com\/products\/hsa-mir-181a-5p-mimic","provider":"알앤디메이트","version":"1.0","type":"link"}