{"product_id":"human-anti-hpf4-anti-heparin-platelet-factor-4-antibody-elisa-kit-elk8037","title":"Human Anti-HPF4(Anti-Heparin\/Platelet Factor 4 Antibody) ELISA Kit","description":"\u003cp\u003eHuman Anti-HPF4(Anti-Heparin\/Platelet Factor 4 Antibody) ELISA Kit\u003c\/p\u003e\n\u003cdiv class=\"item item1\"\u003e\n\u003ch4 class=\"tit clearfix tabs\"\u003e\u003cspan class=\"fz20 fl tran300 active\"\u003eOverview\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle\"\u003e\n\u003cdiv id=\"tabs-container\" class=\"container1 swiper-container-horizontal swiper-container-autoheight\"\u003e\n\u003cdiv class=\"swiper-wrapper\"\u003e\n\u003cdiv class=\"swiper-slide pageStyle fz16 swiper-no-swiping swiper-slide-active\"\u003e\n\u003ctable class=\"table2\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eProduct name:\u003c\/td\u003e\n\u003ctd\u003eHuman Anti-HPF4(Anti-Heparin\/Platelet Factor 4 Antibody) ELISA Kit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"208px\" class=\"fz18\"\u003eReactivity:\u003c\/td\u003e\n\u003ctd\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAlternative Names:\u003c\/td\u003e\n\u003ctd\u003eHPF4 Ab\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Type:\u003c\/td\u003e\n\u003ctd\u003eCompetitive Inhibition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSensitivity:\u003c\/td\u003e\n\u003ctd\u003e0.59 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eStandard:\u003c\/td\u003e\n\u003ctd\u003e100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eDetection Range:\u003c\/td\u003e\n\u003ctd\u003e1.57-100 ng\/mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eSample Type:\u003c\/td\u003e\n\u003ctd\u003eSerum, plasma, tissue homogenates and other biological fluids.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eAssay Length:\u003c\/td\u003e\n\u003ctd\u003e2.5h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eResearch Area:\u003c\/td\u003e\n\u003ctd\u003eHematology;\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd class=\"fz18\"\u003eTest principle:\u003c\/td\u003e\n\u003ctd\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Anti-HPF4 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Anti-HPF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Anti-HPF4 in the samples is then determined by comparing the OD of the samples to the standard curve.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item2\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eStandard curve\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"left fl\"\u003e\n\u003ctable class=\"table3\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration (ng\/mL)\u003c\/th\u003e\n\u003cth\u003eOD\u003c\/th\u003e\n\u003cth\u003eCorrected OD\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e100.00\u003c\/td\u003e\n\u003ctd\u003e0.215\u003c\/td\u003e\n\u003ctd class=\"1\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50.00\u003c\/td\u003e\n\u003ctd\u003e0.275\u003c\/td\u003e\n\u003ctd class=\"2\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e25.00\u003c\/td\u003e\n\u003ctd\u003e0.596\u003c\/td\u003e\n\u003ctd class=\"3\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e12.50\u003c\/td\u003e\n\u003ctd\u003e0.842\u003c\/td\u003e\n\u003ctd class=\"4\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e6.25\u003c\/td\u003e\n\u003ctd\u003e1.139\u003c\/td\u003e\n\u003ctd class=\"5\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e3.13\u003c\/td\u003e\n\u003ctd\u003e1.585\u003c\/td\u003e\n\u003ctd class=\"6\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e1.57\u003c\/td\u003e\n\u003ctd\u003e1.887\u003c\/td\u003e\n\u003ctd class=\"7\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e0.00\u003c\/td\u003e\n\u003ctd\u003e2.157\u003c\/td\u003e\n\u003ctd class=\"8\"\u003e—\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\u003cimg alt=\"\" src=\"https:\/\/www.elkbiotech.com\/upload\/image\/ELISA\/ELK8037-b-1.jpg\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item3\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003ePrecision\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cp\u003eIntra-assay Precision (Precision within an assay)：\u003cspan class=\"point\"\u003eCV%\u0026lt;8%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested twenty times on one plate to assess intra-assay precision.\u003c\/p\u003e\n\u003cp\u003eInter-assay Precision (Precision between assays)：\u003cspan class=\"point\"\u003eCV%\u0026lt;10%\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003eThree samples of known concentration were tested in forty separate assays to assess inter-assay precision.\u003c\/p\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eRecovery\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eMatrices listed below were spiked with certain level of recombinant Anti-HPF4 and the recovery rates were calculated by comparing the measured value to the expected amount of Anti-HPF4 in samples.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth width=\"33.33%\"\u003eRecovery range\u003c\/th\u003e\n\u003cth\u003eAverage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e80-97%\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e88-99%\u003c\/td\u003e\n\u003ctd\u003e93%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e82-95%\u003c\/td\u003e\n\u003ctd\u003e88%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"item item4\"\u003e\n\u003ch4 class=\"tit\"\u003e\u003cspan class=\"fz20\"\u003eLinearity\u003c\/span\u003e\u003c\/h4\u003e\n\u003cdiv class=\"cont fz16 pageStyle clearfix\"\u003e\n\u003cdiv class=\"text fl\"\u003eThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Anti-HPF4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\u003c\/div\u003e\n\u003cdiv class=\"right fr\"\u003e\n\u003ctable class=\"table4\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth width=\"33.33%\"\u003eMatrix\u003c\/th\u003e\n\u003cth\u003e1:2\u003c\/th\u003e\n\u003cth\u003e1:4\u003c\/th\u003e\n\u003cth\u003e1:8\u003c\/th\u003e\n\u003cth\u003e1:16\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eserum(n=5)\u003c\/td\u003e\n\u003ctd\u003e95-104%\u003c\/td\u003e\n\u003ctd\u003e93-102%\u003c\/td\u003e\n\u003ctd\u003e79-94%\u003c\/td\u003e\n\u003ctd\u003e87-98%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEDTA plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e86-93%\u003c\/td\u003e\n\u003ctd\u003e91-105%\u003c\/td\u003e\n\u003ctd\u003e87-98%\u003c\/td\u003e\n\u003ctd\u003e89-94%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eHeparin plasma(n=5)\u003c\/td\u003e\n\u003ctd\u003e86-97%\u003c\/td\u003e\n\u003ctd\u003e93-102%\u003c\/td\u003e\n\u003ctd\u003e87-98%\u003c\/td\u003e\n\u003ctd\u003e83-97%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96T \/ 0.59 ng\/mL \/ 1.57-100 ng\/mL","offer_id":42135231922400,"sku":"ELK8037","price":458.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/products\/ELK_c4c2ce93-923e-4e41-9420-efe4d4b94b37.jpg?v=1638296442","url":"https:\/\/rndmate.com\/products\/human-anti-hpf4-anti-heparin-platelet-factor-4-antibody-elisa-kit-elk8037","provider":"알앤디메이트","version":"1.0","type":"link"}