{"product_id":"hyaluronic-acid-elisa-kit-a75464","title":"Hyaluronic Acid ELISA Kit (A75464)","description":"\u003cdiv id=\"specifications\" class=\"container anchor\"\u003e\n\u003cdiv class=\"bg-light box-shadow-lg rounded-lg\"\u003e\n\u003cdiv class=\"px-4 pb-3 mb-5\"\u003e\n\u003cdiv class=\"tab-content pr-lg-3\"\u003e\n\u003cdiv role=\"tabpanel\" id=\"overview\" class=\"tab-pane fade show active\"\u003e\n\u003cdiv class=\"row\"\u003e\n\u003cdiv class=\"col-lg-6 col-md-6\"\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eName\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eHyaluronic Acid ELISA Kit\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eDescription\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eHyaluronic Acid ELISA Kit is a competitive Enzyme-Linked Immunosorbent Assay (cELISA) designed for the in vitro quantitative determination of Hyaluronic Acid in serum, plasma, tissue homogenates, and other biological fluids.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eAssay Type\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eCompetitive (quantitative)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003ePrinciple of Assay\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eHyaluronic Acid ELISA Kit (A75464) employs the competitive enzyme immunoassay technique for the quantitative measurement of universal Hyaluronic Acid in serum, plasma, tissue homogenates, and other biological fluids. The 96-well microtiter plate has been pre-coated with Hyaluronic Acid antigen. During the incubation, Hyaluronic Acid present in the samples or standards competes with the fixed amount of immobilized Hyaluronic Acid for binding sites on the Biotinylated Anti-Hyaluronic Acid Antibody. The more Hyaluronic Acid present in a sample or standard, the less Biotinylated Anti-Hyaluronic Acid Antibody that binds to the plate. Following incubation, unbound Biotinylated Anti-Hyaluronic Acid Antibody is removed by washing, and an HRP-Streptavidin conjugate is added to the wells and the microtiter plate is incubated. Following incubation and washing, TMB substrate solution is then used to visualize the HRP enzymatic reaction by catalysis to produce a blue-coloured product that changes to yellow after addition of acidic stop solution. The density of yellow is inversely proportional to the amount of Hyaluronic Acid present in each sample or standard. The concentration of Hyaluronic Acid can then be calculated by reading the O.D. absorbance at 450nm in a microplate reader and referring to the standard curve.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003ePlatform\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003ePre-coated Microplate (12 x 8 well strips)\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eDetection Type\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eColorimetric\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eInstrument\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eColorimetric Microplate Reader\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eSample Type\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eSerum, plasma, tissue homogenates, and other biological fluids.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom-small py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eSensitivity\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003e0.938 ng\/ml\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"col-lg-6 col-md-6\"\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eProduct Range\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003e1.563-100 ng\/ml\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eAssay Time\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003e4h 30m\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eReactivity\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eUniversal\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eRecovery\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"table-responsive py-3\"\u003e\n\u003ctable class=\"border font-size-sm table-responsive\"\u003e\n\u003cthead class=\"bg-secondary py-2 border-bottom\"\u003e\n\u003ctr class=\"font-weight-500\"\u003e\n\u003cth class=\"py-2 px-3\"\u003eSample Type\u003c\/th\u003e\n\u003cth class=\"py-2\"\u003en\u003c\/th\u003e\n\u003cth class=\"py-2\"\u003eRange\u003c\/th\u003e\n\u003cth class=\"py-2\"\u003eAverage\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"border-bottom\"\u003e\n\u003ctd class=\"py-2 px-3\"\u003eSerum\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e91% - 104%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e98%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"border-bottom\"\u003e\n\u003ctd class=\"py-2 px-3\"\u003eEDTA Plasma\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e85% - 103%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e94%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"border-bottom\"\u003e\n\u003ctd class=\"py-2 px-3\"\u003eHeparin Plasma\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e89% - 105%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e95%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eLinearity\u003c\/div\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"table-responsive py-3\"\u003e\n\u003ctable class=\"border font-size-sm table-responsive\"\u003e\n\u003cthead class=\"bg-secondary py-2 border-bottom\"\u003e\n\u003ctr class=\"font-weight-500\"\u003e\n\u003cth class=\"py-2 px-3\"\u003eSample Type\u003c\/th\u003e\n\u003cth class=\"py-2\"\u003en\u003c\/th\u003e\n\u003cth class=\"py-2\"\u003e1:2\u003c\/th\u003e\n\u003cth class=\"py-2\"\u003e1:4\u003c\/th\u003e\n\u003cth class=\"py-2\"\u003e1:8\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr class=\"border-bottom\"\u003e\n\u003ctd class=\"py-2 px-3\"\u003eSerum\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e88-102%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e89-103%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e90-105%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"border-bottom\"\u003e\n\u003ctd class=\"py-2 px-3\"\u003eEDTA Plasma\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e82-100%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e85-95%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e85-96%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr class=\"border-bottom\"\u003e\n\u003ctd class=\"py-2 px-3\"\u003eHeparin Plasma\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e5\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e84-99%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e82-99%\u003c\/td\u003e\n\u003ctd class=\"py-2\"\u003e80-94%\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003ePrecision\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eIntra- assay CV: \u0026lt; 8%, Inter-assay CV: \u0026lt; 10%\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eGeneral Notes\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eInformation online is representative. Always refer to the Product Manual inside the kit.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eStorage\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eStore at +4°C. Do not use past expiration date!\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"font-size-sm\"\u003e\n\u003cdiv class=\"d-flex justify-content-between border-bottom py-2\"\u003e\n\u003cdiv\u003e\n\u003cdiv class=\"font-weight-semibold text-muted\"\u003eDisclaimer\u003c\/div\u003e\n\u003cdiv class=\"pt-1\"\u003eThis product is for research use only. It is not intended for diagnostic or therapeutic use.\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cdiv class=\"container anchor\"\u003e\n\u003cdiv class=\"bg-light box-shadow-lg rounded-lg\"\u003e\u003c\/div\u003e\n\u003c\/div\u003e","brand":"antibodies.com","offers":[{"title":"Default Title","offer_id":44953188303072,"sku":"A75464","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/A75464_1.jpg?v=1710307844","url":"https:\/\/rndmate.com\/products\/hyaluronic-acid-elisa-kit-a75464","provider":"알앤디메이트","version":"1.0","type":"link"}