{"product_id":"nsj-fy13404-100ul","title":"Phospho-LIPE (pSer853) Antibody \/ Hormone-sensitive lipase \/ HSL, 100ul","description":"\u003cp\u003ePhospho-LIPE (pSer853) antibody targets the phosphorylated form of Hormone-sensitive lipase (LIPE), also known as HSL, at serine 853, a well-characterized regulatory phosphorylation site associated with activation of lipolytic function. LIPE is a cytoplasmic neutral lipase that plays a central role in lipid mobilization by hydrolyzing triglycerides, diacylglycerols, and cholesteryl esters stored within lipid droplets. In metabolic cells, LIPE localizes primarily to the cytoplasm and lipid droplet surface, with phosphorylation-dependent activation in response to hormonal and signaling cues. Detection of phospho-LIPE provides insight into the activated, signal-responsive pool of HSL rather than total protein abundance.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Functionally, phosphorylation of LIPE at serine 853 is linked to kinase-driven signaling pathways that regulate lipolysis during periods of increased energy demand. This modification promotes enzymatic activation and facilitates interaction with lipid droplets, enabling efficient release of free fatty acids. Hormonal stimulation, such as catecholamine signaling, enhances LIPE phosphorylation, while opposing pathways suppress phosphorylation and favor lipid storage. A Phospho-LIPE (pSer853) antibody supports studies examining dynamic regulation of lipolysis and metabolic signaling pathways.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Phosphorylated LIPE plays an important role in adipocytes, skeletal muscle, and steroidogenic tissues, where controlled lipid mobilization is essential for energy production and biosynthetic processes. In adipose tissue, LIPE phosphorylation coordinates lipid breakdown during fasting or stress, while in muscle it contributes to fuel availability during energy demand. Monitoring phospho-LIPE levels enables assessment of pathway activation states rather than total enzyme expression, providing a functional readout of lipid metabolic regulation.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;From a biological and disease-relevance perspective, dysregulation of LIPE phosphorylation is associated with altered lipid handling, insulin resistance, and metabolic disease. Aberrant signaling that affects LIPE activation can lead to impaired fatty acid release or excessive lipid accumulation. Phospho-LIPE therefore represents a valuable marker for studying signaling pathways that control lipid metabolism, metabolic flexibility, and hormone-responsive energy balance.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;At the molecular level, LIPE is encoded by the LIPE gene and produces a cytoplasmic enzyme that migrates at approximately 80-90 kDa on SDS-PAGE under reducing conditions. Phosphorylation-dependent regulation of LIPE occurs in response to cellular metabolic state and upstream kinase activity. A Phospho-LIPE (pSer853) antibody supports research applications focused on lipid signaling, metabolic regulation, and post-translational control of hormone-sensitive lipase, with NSJ Bioreagents providing reagents intended for research use.\u003c\/p\u003e\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003ctable style=\"width:100%;border-collapse:collapse;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eCatalog No\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eFY13404\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFamily\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003ePrimary antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eQty\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e100ul\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFormat\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eLiquid\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eClone\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eFDO-12\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eHost Animal\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eClonality\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRecombinant Rabbit Monoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eIsotype\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit IgG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eHuman, Mouse, Rat\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eWB, IHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication Details\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eWestern Blot: 1:500-1:2000,Immunohistochemistry: 1:50-1:200\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication Note\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eOptimal dilution of the Phospho-LIPE (pSer853) antibody should be determined by the researcher.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eStatus\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eAvailable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003ePDF Link\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e\u003ca href=\"http:\/\/www.nsjbio.com\/tds-pdf\/phospho-lipe-pser853-antibody-hsl-hormone-sensitive-lipase-fy13404-nsj-bioreagents\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eProduct Data Sheet (PDF)\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eBuffer\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eLimitation\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eThis Phospho-LIPE (pSer853) antibody is available for research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eUniprot #\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eQ05469\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eAffinity chromatography\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eImmunogen\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eA synthesized peptide derived from human Phospho-Hormone-sensitive lipase (pS853) was used as the immunogen for the Phospho-LIPE (pSer853) antibody.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eStore the Phospho-LIPE antibody at -20oC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eLocalization\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eCytoplasm, cell membrane\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eTitle\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003ePhospho-LIPE (pSer853) Antibody \/ Hormone-sensitive lipase \/ HSL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100ul","offer_id":67808535478496,"sku":"FY13404","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/get_image_6e49f7c6-136d-49b4-93f9-f0109fdb8922.jpg?v=1791447812","url":"https:\/\/rndmate.com\/products\/nsj-fy13404-100ul","provider":"알앤디메이트","version":"1.0","type":"link"}