{"product_id":"nsj-v5908-100ug","title":"GST pi Antibody \/ Glutathione S-transferase pi 1, 100ug","description":"\u003cp\u003eGlutathione S-Transferase Pi 1 (GSTP1) is a cytoplasmic detoxification enzyme that belongs to the pi class of glutathione S-transferases, a family of phase II metabolic enzymes that catalyze the conjugation of reduced glutathione to electrophilic substrates. GSTP1 (GSTP1) plays a central role in cellular defense against oxidative stress, xenobiotics, and chemotherapeutic agents by facilitating detoxification and maintaining intracellular redox balance. The GSTP1 Antibody Knockdown Validated \/ Glutathione S-Transferase Pi 1 Antibody is designed to detect this enzyme with high specificity, supported by functional gene silencing validation that directly links signal to target expression. It is part of a collection of \u0026lt;a href=..\/knockdown-validated-antibodies.html\u0026gt;knockdown validated antibodies\u0026lt;\/a\u0026gt; that have been functionally assessed using gene silencing approaches to support target-specific detection.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;GSTP1 antibody, also referred to as Glutathione S-transferase pi 1 antibody and GST pi antibody, recognizes a predominantly cytoplasmic protein that is widely expressed in epithelial tissues and frequently elevated in malignancies. Immunohistochemistry analysis of formalin-fixed, paraffin-embedded human breast carcinoma demonstrates strong HRP-DAB brown cytoplasmic staining in tumor epithelial cells, consistent with the known intracellular localization of GSTP1. The staining pattern highlights clusters of malignant epithelial cells with enhanced metabolic activity, while surrounding stromal components show minimal signal. A negative control inset using PBS in place of primary antibody shows no specific staining, confirming low background and supporting assay specificity.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Western blot analysis reveals a distinct band at approximately 23-25 kDa in human cell lysates, consistent with the expected molecular weight of GSTP1. The signal is sharp and reproducible, supporting reliable detection of endogenous protein across tested samples. Importantly, knockdown validation using GSTP1-targeted shRNA in HeLa cells results in a clear reduction in band intensity compared to wild-type controls, confirming that the detected signal corresponds specifically to GSTP1. This knockdown-based validation provides direct functional evidence of specificity and distinguishes this clone from antibodies validated solely by molecular weight or expression pattern.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Functionally, GSTP1 contributes to detoxification pathways by conjugating glutathione to reactive electrophiles, thereby neutralizing potentially harmful compounds and facilitating their elimination. In addition to its enzymatic role, GSTP1 has been shown to regulate signaling pathways involved in cellular stress responses, including interactions with kinases that influence apoptosis and survival. These dual roles position GSTP1 as both a metabolic enzyme and a modulator of stress-related signaling networks.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;In cancer biology, GSTP1 is frequently overexpressed and has been associated with tumor progression, resistance to chemotherapy, and adaptation to oxidative stress. Elevated GSTP1 levels in tumor epithelial cells reflect enhanced detoxification capacity and survival advantage under cytotoxic conditions. Its cytoplasmic accumulation in carcinoma tissues provides a clear readout of metabolic reprogramming and therapeutic resistance mechanisms. Detection of GSTP1 therefore supports studies of drug resistance, tumor metabolism, and oxidative stress biology in cancer models.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;The combination of strong cytoplasmic immunohistochemical staining in tumor tissue, clear molecular weight detection by western blot, and functional knockdown validation supports the use of clone GSTP1\/12956R for studies of detoxification pathways, redox regulation, and cancer biology. These complementary validation approaches provide confidence in target specificity and make this antibody well-suited for applications requiring robust and reproducible detection.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;This antibody is part of a \u0026lt;a href=..\/antibodies.php\u0026gt;broader antibody panel\u0026lt;\/a\u0026gt; offered by NSJ Bioreagents.\u003c\/p\u003e\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003ctable style=\"width:100%;border-collapse:collapse;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eCatalog No\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eV5908-100UG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFamily\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003ePrimary antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eQty\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e100ug\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e0.2 mg\/ml in 1X PBS with 0.05% BSA, 0.05% sodium azide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFormat\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003ePurified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eClone\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eGSTP1\/12956R\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eHost Animal\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eClonality\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRecombinant Rabbit Monoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eIsotype\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit IgG, kappa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eIHC, WB\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication Details\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eImmunohistochemistry (FFPE): 1-2ug\/ml,Western blot: 2-4ug\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication Note\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eOptimal dilution of the GSTP1 Antibody Knockdown Validated \/ Glutathione S-Transferase Pi 1 Antibody should be determined by the researcher.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eStatus\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eAvailable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003ePDF Link\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e\u003ca href=\"http:\/\/www.nsjbio.com\/tds-pdf\/gstp1-antibody-glutathione-s-transferase-pi-1-gstp1-12956r-v5908\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eProduct Data Sheet (PDF)\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eLimitation\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eThis GSTP1 Antibody Knockdown Validated \/ Glutathione S-Transferase Pi 1 Antibody is available for research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eUniprot #\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eP09211\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003ePurity\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eProtein A affinity\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eImmunogen\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eA recombinant fragment (around amino acids 19-206) of human full-length GSTP1 protein (exact sequence is proprietary) was used as the immunogen for the GSTP1\/Glutathione S-transferase pi 1 antibody.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eGSTP1\/Glutathione S-transferase pi 1 antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eLocalization\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eCytoplasm, Mitochondrion, Nucleus\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eTitle\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eGST pi Antibody \/ Glutathione S-transferase pi 1\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100ug","offer_id":67782942327008,"sku":"V5908-100UG","price":0.0,"currency_code":"KRW","in_stock":true}],"url":"https:\/\/rndmate.com\/products\/nsj-v5908-100ug","provider":"알앤디메이트","version":"1.0","type":"link"}