{"product_id":"nsj-v6139-100ug","title":"CD3 epsilon Antibody, 100ug","description":"\u003cp\u003eCD3 epsilon (CD3E) is a transmembrane component of the T-cell receptor (TCR) complex that is universally expressed in mature T lymphocytes and serves as a defining marker for T-cell identification in tissue sections. CD3e Antibody for IHC is specifically suited for detecting CD3 Epsilon \/ CD3E in formalin-fixed, paraffin-embedded samples, enabling clear visualization of T-cell distribution within intact tissue architecture. CD3e antibody, also known as CD3 epsilon antibody or CD3E antibody, is widely recognized in the literature as a pan-T cell marker antibody due to its consistent and robust expression across T-cell populations.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Within the TCR complex, CD3 epsilon associates with CD3 gamma, CD3 delta, and CD3 zeta chains to form a signaling-competent receptor that mediates antigen recognition and downstream activation. This functional role underlies its strong and stable expression in T cells across lymphoid and peripheral tissues. As a result, CD3e antibody reagents are foundational tools for visualizing adaptive immune components directly in tissue sections, where preservation of morphology is critical for interpretation.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;In immunohistochemistry, CD3e Antibody for IHC provides distinct membranous and cytoplasmic staining in T lymphocytes, allowing precise identification of T-cell populations within complex tissue environments. This staining pattern enables clear separation of T cells from surrounding epithelial, stromal, and other immune cell types. In normal tissues such as tonsil, lymph node, and thymus, CD3 epsilon immunohistochemistry highlights dense T-cell zones including interfollicular regions and paracortical areas, while in non-lymphoid tissues it reveals scattered resident or infiltrating T cells within mucosal and stromal compartments.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;This CD3e Antibody for IHC is uniquely positioned for tissue-based immune profiling through extensive validation on tissue microarrays. Using clone MSVA-003R, a recombinant rabbit monoclonal antibody, consistent and high-contrast staining is observed across a broad panel of normal human tissues. The TMA format enables side-by-side comparison of staining patterns across organ systems, demonstrating expected localization of CD3-positive lymphocytes while maintaining low background in non-target cells. This level of cross-tissue consistency reinforces the reliability of the antibody for large-scale tissue studies and comparative pathology applications.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;The inclusion of cancer tissue microarrays further strengthens the utility of this CD3e antibody for evaluating tumor-associated immune responses. Across multiple tumor types, CD3 epsilon staining highlights tumor-infiltrating lymphocytes within stromal compartments and at invasive margins, providing clear visualization of immune cell distribution relative to tumor architecture. Variability in T-cell density across tumor samples is readily detectable, supporting studies of immune heterogeneity and tumor microenvironment characterization. The ability to detect these patterns reproducibly across TMA panels makes this CD3e Antibody for IHC particularly valuable for high-throughput tissue analysis.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;Clone MSVA-003R has been developed to deliver strong, specific staining in FFPE tissues, with performance characteristics that support consistent interpretation across both normal and diseased samples. Its recombinant rabbit monoclonal format contributes to reproducible signal intensity and low non-specific background, which are critical for immunohistochemical applications. By combining robust staining quality with extensive TMA validation, this CD3e Antibody for IHC serves as a reliable tool for identifying T cells and mapping immune landscapes in tissue-based research settings.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;A full range of CD3e antibody reagents for immunohistochemistry, western blot, and flow cytometry is available on our \u0026lt;a href=..\/cd3e-antibody-cd3-epsilon-antibodies.html\u0026gt;CD3e Antibody\u0026lt;\/a\u0026gt; collection page.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;This antibody is also part of a broader collection of \u0026lt;a href='\/ihc-antibodies-tissue-microarray-validated.html'\u0026gt;IHC antibodies validated by tissue microarray analysis\u0026lt;\/a\u0026gt;, supporting consistent staining across normal and cancer tissues.\u003c\/p\u003e\u003ch3\u003eSpecifications\u003c\/h3\u003e\u003ctable style=\"width:100%;border-collapse:collapse;\"\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eCatalog No\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eV6139-100UG\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFamily\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003ePrimary antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eQty\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e100ug\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFormulation\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eAntibody in 1X PBS with 0.05% BSA, 0.05% sodium azide\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eFormat\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003ePurified\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eClone\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eMSVA-003R\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eHost Animal\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eClonality\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRecombinant Rabbit Monoclonal\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eIsotype\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eRabbit IgG, kappa\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eHuman\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eIHC\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication Details\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eImmunohistochemistry (FFPE): 1-2ug\/ml\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eApplication Note\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e1. Optimal dilution of the CD3e Antibody for IHC \/ CD3 Epsilon Immunohistochemistry Antibody should be determined by the researcher. \u0026lt;br\u0026gt;\u0026lt;br\u0026gt;2. This CD3E \/ CD3 epsilon antibody is recombinantly produced by expression in human HEK293 cells.\u0026lt;br\u0026gt;\u0026lt;br\u0026gt;3. Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121oC in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37oC for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eStatus\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eAvailable\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003ePDF Link\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003e\u003ca href=\"http:\/\/www.nsjbio.com\/tds-pdf\/cd3e-antibody-for-ihc-cd3-epsilon-immunohistochemistry-antibody-msva-003r-v6139\" target=\"_blank\" rel=\"noopener noreferrer\"\u003eProduct Data Sheet (PDF)\u003c\/a\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eLimitation\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eThis CD3E \/ CD3 epsilon antibody is available for research use only.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eUniprot #\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eP07766\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eImmunogen\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eSynthetic peptide from human CD3e protein (around amino acids 20-40) (exact sequence is proprietary) was used as the immunogen for the CD3e Antibody for IHC \/ CD3 Epsilon Immunohistochemistry Antibody.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eStorage\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eCD3E \/ CD3 epsilon antibody with sodium azide - store at 2 to 8oC; antibody without sodium azide - store at -20 to -80oC.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eLocalization\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eCell membrane\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth style=\"text-align:left;vertical-align:top;padding:6px;border-bottom:1px solid #eee;\"\u003eTitle\u003c\/th\u003e\n\u003ctd style=\"padding:6px;border-bottom:1px solid #eee;\"\u003eCD3 epsilon Antibody\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e","brand":"NSJ Bioreagents","offers":[{"title":"100ug","offer_id":67782744113376,"sku":"V6139-100UG","price":0.0,"currency_code":"KRW","in_stock":true}],"url":"https:\/\/rndmate.com\/products\/nsj-v6139-100ug","provider":"알앤디메이트","version":"1.0","type":"link"}