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SKU(재고 관리 코드):PAF878Hu01
Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)
Polyclonal Antibody to Secreted Frizzled Related Protein 4 (SFRP4)
Western blotting: 0.5-2µg/mL;1:250-1000
;Immunohistochemistry: 5-20µg/mL;1:25-100
;Immunocytochemistry: 5-20µg/mL;1:25-100
;Optimal working dilutions must be determined by end user.
Product No.
PAF878Hu01
Organism Species
Homo sapiens (Human).
Source
Polyclonal antibody preparation
Host
Rabbit
Potency
n/a
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
None
Immunogen
RPF878Hu01-Recombinant Secreted Frizzled Related Protein 4 (SFRP4)
Buffer Formulation
0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Traits
Liquid
Concentration
0.5mg/ml
Organism Species More
Mus musculus (Mouse), Rattus norvegicus (Rat)
Applications
WB; IHC; ICC; IP.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against SFRP4. It has been selected for its ability to recognize SFRP4 in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.5-2µg/mL;1:250-1000
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Endocrine Journal |
Relationship between serum secreted frizzled-related protein 4 levels and the first-phase of glucose-stimulated insulin secretion in individuals with different glucose tolerance PubMed: 26041556 |
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Horm Metab Res. |
Secreted Frizzled-Related Protein 4 (SFRP4) is Elevated in Patients with Diabetes Mellitus Pubmed:26882051 |
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Obesity (Silver Spring). |
Reduced expression of chemerin in visceral adipose tissue associates with hepatic steatosis in patients with obesity pubmed:27768254 |
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Obesity |
Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans Pubmed:29427376 |
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Novel marker for gestational diabetes patent:US20180059122A1 |
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British Journal Of Nutrition |
A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile Pubmed: 30375290 |
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American Journal of Cancer Research |
SFRP4 is a prognostic marker and correlated with Treg cell infiltration in pancreatic ductal adenocarcinoma Pubmed: 30906634 |
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Diabetologia |
Effects of long-term exercise on plasma adipokine levels and inflammation-related gene expression in subcutaneous adipose tissue in sedentary dysglycaemic … Pubmed: 31011777 |
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PLoS One |
First trimester secreted Frizzled-Related Protein 4 and other adipokine serum concentrations in women developing gestational diabetes mellitus Pubmed: 33206702 |
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Gastric Cancer |
SFRP4 drives invasion in gastric cancer and is an early predictor of recurrence 33277667 |
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Diabetology |
Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop … |
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Catalog No. |
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Applications (RESEARCH USE ONLY!) |
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WB,IHC |
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WB; IHC; ICC; IP. |
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Enzyme-linked immunosorbent assay for Antigen Detection. |
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