{"product_id":"recombinant-human-pdgf-b-p4397","title":"Recombinant Human PDGF B","description":"\u003cp\u003e\u003cstrong\u003eProduct Name\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eRecombinant Human pdgf-A\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSize\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e50μg, 200μg, 1mg\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eSpecies\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eHuman\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eHost\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eE.Coli\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eExpression Region\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e87-211\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMol. Weight\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e16.2 kDa\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eTags\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eN-terminal His Tag\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003ePurity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eGreater than 90% as determined by SDS-PAGE\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eApplication\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eWestern Blot, ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eActivity\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eMeasured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells.The ED50 for this effect is 60-230 ng\/mL in a fluorometric assay.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eFormulation\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eLyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH7.4\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eReconstitution\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eCentrifuge the vial prior to opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1mg\/ml by gently pipetting 2-3 times, don't vortex\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eStorage\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eThe lyophilized protein is stable at -20°C for up to 1 year, the protein solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing 0.1% BSA (make sure BSA will NOT interfere with your experiment ) and store in working aliquots at -20°C to -80°C for 6 months.Avoid repeated freeze\/thaw cycle.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAlternative Names\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePDGF A, PDGF A chain, PDGF subunit A, PDGF-1, PDGF1, PDGFA, PDGFA_HUMAN, Platelet derived growth factor alpha chain, Platelet derived growth factor alpha isoform 2 preproprotein, Platelet derived growth factor alpha polypeptide, Platelet-derived growth factor A chain\u003c\/p\u003e\n\u003cp\u003e \u003c\/p\u003e","brand":"FineTest","offers":[{"title":"82-190 \/ 11.9 kDa \/ 200ug","offer_id":42523748303072,"sku":"P4397","price":626.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/products\/fine_test_logo_df2077cf-20a4-4a63-913d-0cc04480def6.jpg?v=1646931999","url":"https:\/\/rndmate.com\/products\/recombinant-human-pdgf-b-p4397","provider":"알앤디메이트","version":"1.0","type":"link"}