{"product_id":"trastuzumab-herceptin-pharmacokinetic-elisa-el-1611-201","title":"Trastuzumab (Herceptin) Pharmacokinetic ELISA","description":"\u003ch2\u003eTrastuzumab (Herceptin) Pharmacokinetic ELISA\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eAffinityImmuno Trastuzumab (Herceptin) Pharmacokinetic ELISA\u003c\/strong\u003e is a ready-to-use ELISA kit designed for pharmacokinetic (PK), anti-drug antibody (ADA), and immunogenicity workflows in biologics and biosimilar development.\u003c\/p\u003e\n\u003cp\u003eTrastuzumab (Herceptin®) is indicated for the treatment of HER2-positive breast cancer, and adjuvant therapies for metastatic gastric cancer and gastroesophageal cancer. Serum concentration of Trastuzumab may predict some clinical outcome during therapy. It is also possible that the surveillance of circulating Trastuzumab concentration during therapy represents a direct factor for immunogenicity and some other side effects. Identification of biomarkers and risk factors for adverse drug reactions that might be related to serum concentrations, and maintaining the effective minimum concentration of Trastuzumab in order to potentially avoid some side effects, might be beneficial using a reliable method.\u003c\/p\u003e\n\u003ch3\u003eProduct Highlights\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget Drug:\u003c\/strong\u003e Trastuzumab (Herceptin)\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct Type:\u003c\/strong\u003e ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplication:\u003c\/strong\u003e Quantification of Trastuzumab in biological matrices\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMethod:\u003c\/strong\u003e Direct sandwich ELISA\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Method:\u003c\/strong\u003e Peroxidase \/ OD450\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Human, mouse, rat\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Range:\u003c\/strong\u003e 40ng\/ml - 1.25ng\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eDetection Minimum:\u003c\/strong\u003e 1.25ng\/ml\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Type:\u003c\/strong\u003e Serum Plasma\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Volume:\u003c\/strong\u003e 15ul\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Time:\u003c\/strong\u003e 2.5 hours\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eStorage:\u003c\/strong\u003e -20C, 1 year\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eShipping:\u003c\/strong\u003e dry ice, less than -20C\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePack Size:\u003c\/strong\u003e 1X96 wells\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity:\u003c\/strong\u003e Trastuzumab\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePlate Format:\u003c\/strong\u003e Strip\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003eKit Details\u003c\/h3\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eComponents:\u003c\/strong\u003e Coated microtiter plate, 96 wells\nCalibrator diluent. - 1.8ml\nCalibrator 12ul\n10X wash buffer - 25ml\nAssay buffer - 50ml\n1000X detection reagent - 17ul\nTMB - 12ml\nTMB stop solution - 12ml\nPlate sealers - 3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Procedure:\u003c\/strong\u003e This assay employs the sandwich enzyme immunoassay technique. Anti- Trastuzumab is coated onto a 96 well microplate. Calibrator, quality control samples (if desired) and test samples are pipetted into the appropriate wells. Trastuzumab present in biological matrices is bound by the immobilized anti- Trastuzumab antibody. After washing away any unbound substances, enzyme linked antiTrastuzumab antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Trastuzumab present in test samples. The color development is stopped and the intensity of the color is measured.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReagent Preparation:\u003c\/strong\u003e Prepare only the appropriate amount of required reagent on the day of use. Store all reagents as per instructions stated on the label. 1. Wash Buffer (1X) Preparation: Dilute wash buffer concentrate with ultra-pure water 1\/10 before use (for example add 10mL concentrate to 90mL ultra-pure water). Mix well. 2. Detection Reagent (1X) Preparation: Dilute detection reagent with assay buffer 1\/1000 before use (for example add 10μl concentrate to 10ml of assay buffer). Mix welll. 3. Preparation of Calibrators: Prepare calibrators with concentrations ranging from 1000 ng\/mL to 62.5 ng\/mL. The following is an example calibrator curve.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eResults Calculation:\u003c\/strong\u003e 1. Construct a standard curve by plotting the absorbance obtained from each standard against concentration. Use a 4 or 5 parameter curve fit. Alternatively a log-log curve fit may be used. 2. The concentration of the unknowns can be read directly from this standard curve using the absorbance value for each sample. 3. Any sample undiluted or diluted still reading greater than the highest standard should be diluted appropriately with calibrator diluent and retested. If the samples have been diluted, the concentration determined from the standard curve must be multiplied by the dilution factor.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Collection:\u003c\/strong\u003e This kit is compatible with EDTA-plasma, heparinplasma and serum samples. Samples can be stored at or below -20°C for up to 1 year.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSample Preparation:\u003c\/strong\u003e Dilute calibrators and test samples 1\/50 with assay buffer (for example add 5µL of prepared calibrator or sample to 245µL of assay buffer). Mix well. Do not store diluted samples.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAssay Precision:\u003c\/strong\u003e \u0026lt;10%, \u0026lt;10%\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePreservative:\u003c\/strong\u003e None\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGene ID:\u003c\/strong\u003e 2064\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003e\u003cstrong\u003eSEO Keywords:\u003c\/strong\u003e Trastuzumab (Herceptin), Trastuzumab (Herceptin) Pharmacokinetic ELISA, AffinityImmuno, ELISA, PK assay, ADA assay, biologics analysis, immunogenicity, bioanalytical testing, pharmacokinetic ELISA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eDatasheet:\u003c\/strong\u003e \u003ca href=\"http:\/\/affinityimmuno.com\/wp-content\/uploads\/2016\/10\/EL-1611-201-Herceptin-PK-PRODUCT-SHEET.pdf\"\u003ehttp:\/\/affinityimmuno.com\/wp-content\/uploads\/2016\/10\/EL-1611-201-Herceptin-PK-PRODUCT-SHEET.pdf\u003c\/a\u003e\u003c\/p\u003e","brand":"Affinity Immuno","offers":[{"title":"Default Title","offer_id":50127972237536,"sku":"EL-1611-201","price":0.0,"currency_code":"KRW","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0601\/9330\/8896\/files\/AffinityImmuno-Product-3_ea90b399-e11d-4e55-8cd3-a94b73b9c9d4.jpg?v=1773910665","url":"https:\/\/rndmate.com\/products\/trastuzumab-herceptin-pharmacokinetic-elisa-el-1611-201","provider":"알앤디메이트","version":"1.0","type":"link"}