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SKU(재고 관리 코드):SEA193Mu

ELISA Kit for Fibrinogen (FG)

ELISA Kit for Fibrinogen (FG)

UOM
Sensitivity
Detection range

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

SEA193Mu

Organism Species

Mus musculus (Mouse).

Sample Type

plasma

Test Method

Double-antibody Sandwich

Assay Length

3h

Detection Range

6.25-400ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 2.61ng/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Fibrinogen (FG).
No significant cross-reactivity or interference between Fibrinogen (FG) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Fibrinogen (FG) and the recovery rates were calculated by comparing the measured value to the expected amount of Fibrinogen (FG) in samples.

Matrix Recovery range (%) Average(%)
sodium citrate plasma(n=5) 83-97 91

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Fibrinogen (FG) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Fibrinogen (FG) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Fibrinogen (FG) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
sodium citrate plasma(n=5) 85-96% 92-103% 95-103% 92-101%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Magazine Citations
Chemical Research in Toxicology Sub-chronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwall Carbon Nanotubes by Intratracheal Instillation Pubmed:25580880
Chemical Research in Toxicology Subchronic Toxicity and Cardiovascular Responses in Spontaneously Hypertensive Rats after Exposure to Multiwalled Carbon Nanotubes by Intratracheal Instillation PubMed: 25580880
VETERINARSKI ARHIV Biomarkers of ketosis in dairy cows at postparturient period: acute phase proteins and pro-inflammatory cytokines doi: 10.24099/vet.arhiv.160126c
VETERINARSKI ARHIV Biomarkeri ketoze u mliječnih krava u postpartalnom razdoblju: proteini akutne faze i proupalni citokini doi: 10.24099/vet.arhiv.160126c
Spike protein up-regulates inflammatory axis of both thromboinflammation and leukotriene in severe COVID-19
Toxicol Appl Pharmacol CREB1 protects against the renal injury in a rat model of kidney stone disease and calcium oxalate monohydrate crystals-induced injury in NRK-52E cells 33421503
large animal review Influence of niacin application on inflammatory parameters, non-esterified fatty acids and functional status of liver in cows during early lactation
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
NPA193Mu01 Native Fibrinogen (FG) Positive Control; Immunogen; SDS-PAGE; WB.
SEA193Mu ELISA Kit for Fibrinogen (FG) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA193Mu Magnetic Luminex Assay Kit for Fibrinogen (FG) ,etc. Magnetic Luminex Assay for Antigen Detection.
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