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SKU(재고 관리 코드):SEC643Mu

ELISA Kit for Monoamine Oxidase B (MAOB)

ELISA Kit for Monoamine Oxidase B (MAOB)

UOM
Sensitivity
Detection range

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

SEC643Mu

Organism Species

Mus musculus (Mouse).

Sample Type

serum, plasma, tissue homogenates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

3h

Detection Range

31.2-2,000pg/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 12.2pg/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Monoamine Oxidase B (MAOB).
No significant cross-reactivity or interference between Monoamine Oxidase B (MAOB) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Monoamine Oxidase B (MAOB) and the recovery rates were calculated by comparing the measured value to the expected amount of Monoamine Oxidase B (MAOB) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 79-88 80
EDTA plasma(n=5) 78-104 93
heparin plasma(n=5) 86-104 92

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Monoamine Oxidase B (MAOB) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Monoamine Oxidase B (MAOB) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Monoamine Oxidase B (MAOB) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 95-102% 78-94% 92-102% 95-102%
EDTA plasma(n=5) 89-104% 97-105% 88-102% 88-102%
heparin plasma(n=5) 78-97% 79-93% 78-98% 80-103%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Magazine Citations
Neurotoxicology. Neuroprotective effect of thalidomide on MPTP-induced toxicity Pubmed:25724264
brain research Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. pubmed:28238669
researchsquare Aging and MPTP-Sensitivity Depend on Molecular and Ultrastructural Signatures of Astroglia and Microglia in Mice Substantia Nigra
Catalog No. Related products for research use of Mus musculus (Mouse) Organism species Applications (RESEARCH USE ONLY!)
RPC643Mu01 Recombinant Monoamine Oxidase B (MAOB) Positive Control; Immunogen; SDS-PAGE; WB.
PAC643Mu01 Polyclonal Antibody to Monoamine Oxidase B (MAOB) WB; IHC; ICC; IP.
SEC643Mu ELISA Kit for Monoamine Oxidase B (MAOB) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC643Mu Magnetic Luminex Assay Kit for Monoamine Oxidase B (MAOB) ,etc. Magnetic Luminex Assay for Antigen Detection.
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