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Cloud-clone

SKU(재고 관리 코드):SCA063Hu

CLIA Kit for Interleukin 17 (IL17)

CLIA Kit for Interleukin 17 (IL17)

UOM
Sensitivity
Detection range

Chemiluminescent immunoassay for Antigen Detection.

Product No.

SCA063Hu

Organism Species

Homo sapiens (Human).

Sample Type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

2h, 40min

Detection Range

1.37-1,000pg/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.53pg/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

 

Specificity

This assay has high sensitivity and excellent specificity for detection of Interleukin 17 (IL17).
No significant cross-reactivity or interference between Interleukin 17 (IL17) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Interleukin 17 (IL17) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 17 (IL17) in samples.

Matrix Recovery range (%) Average(%)
serum(n=5) 87-104 101
EDTA plasma(n=5) 93-101 97
heparin plasma(n=5) 95-103 101

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 17 (IL17) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 17 (IL17) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample 1:2 1:4 1:8 1:16
serum(n=5) 78-94% 86-104% 98-105% 92-99%
EDTA plasma(n=5) 78-93% 90-97% 93-105% 82-95%
heparin plasma(n=5) 78-104% 88-103% 89-96% 94-102%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
Substrate A 1×10mL Substrate B 1×2mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 100µL Substrate Solution. Incubate 10 minutes at 37°C;
8. Read RLU value immediately.

Magazine Citations
PLOS ONE Impaired Autoimmune T Helper 17 Cell Responses Following DNA Vaccination against Rat Experimental Autoimmune Encephalomyelitis PubMed: PMC2577303
Cellular Immunology Atorvastatin ameliorates experimental autoimmune neuritis by decreased Th1/Th17 cytokines and up-regulated T regulatory cells ScienceDirect: S0008874911002164
The Journal of Urology Improvement by Phytotherapeutic Agent of Detrusor Overactivity, Down-Regulation of Pharmacological Receptors and Urinary Cytokines in Rats with Cyclophosphamide Induced Cystitis PubMed: S0022534712049087
Critical Care Medicine Systemic release of cytokines and heat shock proteins in porcine models of polytrauma and hemorrhage PubMed: PMC3383056
Lower Urinary Tract Symptoms Effects of Saw Palmetto Extract on Urodynamic Parameters, Bladder Muscarinic and Purinergic Receptors and Urinary Cytokines in Rats with Cyclophosphamide-Induced Cystitis Onlinelibrary: luts.12015
Shock Expression of Hemopexin in Acute Rejection of Rat Liver Allograft Identified by Serum Proteomic Analysis. Lww: Source
J Vet Med Sci. CD4+ T Cell Cytokine Gene and Protein Expression in Duodenal Mucosa of Dogs with Inflammatory Bowel Disease Pubmed:24270804
Journal of Dairy Science Increased blood-circulating interferon-γ, interleukin-17, and osteopontin levels in bovine paratuberculosis Pubmed:24679935
Inflammation.? Pemetrexed Ameliorates Experimental Arthritis in Rats Pubmed:25129058
Inflammation Lapatinib Ameliorates Experimental Arthritis in Rats Pubmed:25283886
Molecular and Cellular Biochemistry Beta-arrestin-2 negatively modulates inflammation response in mouse chondrocytes induced by 4-mer hyaluronan oligosaccharide Pubmed:25318610
Molecular Medicine Reports Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis Pubmed:25323532
Genet Mol Res Protective effect and mechanism of probucol in the treatment of spinal cord injury PubMed: 26214485
veterinary research forum Evaluation of proliferation and cytokines production by mitogen-stimulated bovine peripheral blood mononuclear cells Article_14936_6.Html:
Tumor Biology Hypoxia-induced secretion of IL-20 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma PubMed: 26695151
Clinical & Experimental Immunology MicroRNA‐155 may be involved in the pathogenesis of atopic dermatitis by modulating the differentiation and function of T helper type 17 (Th18) cells PubMed: 25761610
J Reprod Immunol The immunomodulating effect of seminal plasma on T cells PubMed: 25799173
Biomarkers Interleukin-17 levels correlate with poor prognosis and vascular endothelial growth factor concentration in the serum of patients with non-small cell lung cancer PubMed: 26301881
Molecular Medicine Reports Tanshinone IIA attenuates experimental autoimmune encephalomyelitis in rats Pubmed:27357729
SpringerPlus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression ofHMGB1/RAGE/NF-ΚB pathway. Pubmed:27026909
International Immunopharmacology Consistency and pathophysiological characterization of a rat polymicrobial sepsis model via the improved cecal ligation and puncture surgery Pubmed:26802602
Journal of Agricultural Biotechnology Gln Attenuates LPS-induced Inflammatory Injury of Bos taurus Testis Sustentacular Cells 10610
Veterinary Research Communications Immunological and pathological investigations in equine experimental uveitis pubmed:27344152
Journal of Neuroinflammation Th17-skewed immune response and cluster of differentiation 40 ligand expression in canine steroid-responsive meningitis-arteritis, a large animal model for neutrophilic meningitis. pubmed:28114998
American Journal of Translational Research Anti-inflammatory effects of Boletus edulis polysaccharide on asthma pathology pubmed:27830033
Springerplus Ethyl pyruvate attenuated coxsackievirus B3-induced acute viral myocarditis by suppression of HMGB1/RAGE/NF-ΚB pathway pubmed:27026909
Journal of Virology Characterization of Simian Immunodeficiency Virus Variants Anatomically Compartmentalized inPlasma and Milk in Chronically Infected African Green Monkeys. pubmed:
J Nephropathol Relationship between IL-17 serum level and ambulatory blood pressure in women with polycystic ovary syndrome pubmed:28042549
Journal of Nephropathology Relationship between IL-17 and ambulatory blood pressure in polycystic ovary syndrome 8b168a09f5832df1b5c2f48631612458b2d8.pdf
Basic Clin Pharmacol Toxicol. Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4+ T Helper 17 (Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling. pubmed:28032440
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice pubmed:28250847
Procalcitonin und proinflammatorische Zytokine in der bronchoalveolären Lavage beim lungenkranken Pferd unter inhalativer Glukokortikoidtherapie FUDISS_thesis_000000103830
Scientific Report Anti-asthmatic efect of pitavastatin through aerosol inhalation is associated with CD4+ CD25+ Foxp3+ T cells in an asthma mouse model pubmed:28729731
Basic & Clinical Pharmacology & Toxicology Magnolol Attenuates Concanavalin A-induced Hepatic Fibrosis, Inhibits CD4 + T Helper 17(Th17) Cell Differentiation and Suppresses Hepatic Stellate Cell Activation: Blockade of Smad3/Smad4 Signalling doi:10.1111
Academic Journal Antiinflammatory and antioxidant effects of gemcitabine in collagen-induced arthritis model pubmed:28618762
International Journal of Clinical and Experimental Pathology Low-density neutrophils in severe fever with thrombocytopenia syndrome (SFTS) display decreased function to phagocytose SFTS virus and enhanced capacity to synthesize cytokines ISSN:1936-2625/IJCEP0045334
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice pubmed:28122397
MYOPAIN Increased Levels of IL-17, IL-23, MIP-1α, MCP-1 and Global Leukocytes in Fibromyalgia Patients 10.1080/24708593.2017.1357664
Molecular Medicine Reports Lower level of IL‑35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test‑positive hemocytopenia. pubmed:29257310
Molecular Medicine Reports Interleukin-17 antagonist attenuates lung inflammation through inhibition of the ERK1/2 and NF-κB pathway in LPS-induced acute lung injury 10.3892/mmr.2017.6837
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY  Dexamethasone reduces serum level of IL-17 in Bleomycin-A5-induced rats model of pulmonary fibrosis pubmed:28608724
International Immunopharmacology Therapeutic effect of bone marrow mesenchymal stem cells pretreated with acetylsalicylic acid on experimental periodontitis in rats pubmed:29195233
Biomedical Research Associations among interleukin-17, interferon-gamma, and acute coronary syndrome ISSN 0970-938X
Cellular Physiology and Biochemistry Activated Α7nachr Improves Postoperative Cognitive Dysfunction and Intestinal Injury Induced by Cardiopulmonary Bypass in Rats: Inhibition of the Proinflammatory … Pubmed:29672286
Frontiers in Pharmacology Traditional Herbal Medicine-Derived Sulforaphene LFS-01 Reverses Colitis in Mice by Selectively Altering the Gut Microbiota and Promoting Intestinal … Pubmed:29375374
Bioscience Reports Decreased miR-128 expression alleviated the progression of Rheumatoid arthritis by up-regulating the expression of TNFAIP3 Pubmed:29853534
Medical Science Monitor Involvement of T-Helper 9 Activation in a Mouse Model of Allergic Rhinitis Pubmed:29982264
Journal of International Medical Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease Pubmed:29950127
Canadian Journal of Physiology and Pharmacology Inhibition of microRNA-155 attenuates concanavalin A-induced autoimmune hepatitis by regulating Treg/Th17 cell differentiation Pubmed: 30290123
BMC Microbiology Nystatin enhances the immune response against Candida albicans and protects the ultrastructure of the vaginal epithelium in a rat model of vulvovaginal … Pubmed: 30359236
Biology of Reproduction Recombinant adiponectin alleviates abortion in mice by regulating Th17/Treg imbalance via p38MAPK-STAT5 pathway Pubmed: 30496353
BMC MICROBIOLOGY Enhanced antifungal activity of bovine lactoferrin-producing probiotic Lactobacillus casei in the murine model of vulvovaginal candidiasis Pubmed: 30621597
CNS Neuroscience & Therapeutics Negative regulation of glial Tim‐3 inhibits the secretion of inflammatory factors and modulates microglia to antiinflammatory phenotype after experimental intracerebral?… Pubmed: 30677253
OPEN ACCESS MACEDONIAN JOURNAL OF MEDICAL SCIENCES Seluang Fish (Rasbora Spp.) Oil Decreases Inflammatory Cytokines Via Increasing Vitamin D Level in Systemic Lupus Erythematosus
Frontiers in Microbiology Local probiotic Lactobacillus crispatus and Lactobacillus delbrueckii exhibit strong antifungal effects against vulvovaginal candidiasis in a rat model
中国免疫学杂志 TRAF-6 调控TGF-β1-Smad2/Smad3 信号通路在Graves 病免疫发病机制中的作用
Microbial Pathogenesis Therapeutic effectiveness of type I interferon in vulvovaginal candidiasis Pubmed: 31158491
Inflammation Protein Kinase C Theta Inhibition Attenuates Lipopolysaccharide-Induced Acute Lung Injury through Notch Signaling Pathway via Suppressing Th17 Cell Response … Pubmed: 31297750
international immunopharmacology Adenovirus-mediated down-regulation of miR-21-5p alleviates experimental autoimmune uveoretinitis in mice Pubmed: 31228813
Untersuchung einer Th17-vermittelten Immunantwort bei der kaninen idiopathischen Epilepsie
Free Radical Biology and Medicine Vitamin D protects against oxidative stress, inflammation and hepatorenal damage induced by acute paracetamol toxicity in rat Pubmed: 31255668
BioMed Research International Indoleamine 2, 3-Dioxygenase (IDO) Regulates Th17/Treg Immunity in Experimental IgA Nephropathy
Animals Anti-Inflammatory State in Arabian Horses Introduced to the Endurance Training Pubmed: 31462005
World Journal of Pediatrics Regulation of JAK/STAT signal pathway by miR-21 in the pathogenesis of juvenile idiopathic arthritis Pubmed: 31641939
Biotechnology and Applied Biochemistry Epigallocatechin 3‐gallate attenuates arthritis by regulating Nrf‐2, HO‐1 and cytokine levels in an experimental arthritis model Pubmed: 31746064
Microbial Pathogenesis Tangeretin attenuates lipopolysaccharide-induced acute lung injury through Notch signaling pathway via suppressing Th17 cell response in mice Pubmed: 31676364
International Journal of Rheumatic Diseases Effects of thalidomide on Th17, Treg cells and TGF‐β1/Smad3 pathway in a mouse model of systemic sclerosis Pubmed: 31840939
Cytokine Levels of pro-and anti-inflammatory cytokines in cystic fibrosis patients with or without gingivitis Pubmed: 31927460
international urology and nephrology The therapeutic effect of dendritic cells expressing indoleamine 2, 3-dioxygenase (IDO) on an IgA nephropathy mouse model Pubmed: 31894556
JOURNAL OF PERIODONTAL RESEARCH Salivary Del‐1, IL‐17, and LFA‐1 levels in periodontal health and disease Pubmed: 32153040
journal of molecular endocrinology LncRNA Profile in Hashimoto's Thyroiditis and Potential Function of NONHSAT079547. 2 Pubmed: 32229697
Toxicol Appl Pharmacol Daphnetin ameliorates glucocorticoid-induced osteoporosis via activation of Wnt/GSK-3β/β-catenin signaling Pubmed: 33171191
CARDIOVASCULAR DRUGS AND THERAPY Rhodiola crenulata reduces ventricular arrhythmia through mitigating the activation of IL-17 and inhibiting the MAPK signaling pathway Pubmed: 32946036
Biomed Research International Effects of 2, 3, 7, 8-Tetrachlorodibenzo-p-dioxin on T Cell Differentiation in Primary Biliary Cholangitis Pubmed: 32908870
J Neurosurg Covering the proximal nerve stump with chondroitin sulfate proteoglycans prevents traumatic painful neuroma formation by blocking axon regeneration after … Pubmed: 32470939
JOURNAL OF MICROBIOLOGY Melatonin attenuates microbiota dysbiosis of jejunum in short-term sleep deprived mice Pubmed: 32424577
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat Pubmed: 32395767
Journal of Biochemical and Molecular Toxicology Vitamin D3 and calcium cosupplementation alleviates cadmium hepatotoxicity in the rat: Enhanced antioxidative and anti‐inflammatory actions by remodeling cellular …
Revista da Associacao Medica Brasileira A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic …
biosciences biotechnology research asia Dietary Malted Barley Grain Improved Structure and Function of Spinal Cord of Mother Rats fed on a high cholesterol diet.
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction
Effects of minocycline on neuroinflammation, gut microbiome and hippocampal neurogenesis in rats with Gulf War Illness
Minocycline Alleviate A Rat Model Of Gulf War Illness Via Altering Gut Microbiome, Attenuating Neuroinflammation And Enhancing Hippocampal Neurogenesis
中国应用生理学杂志 丹参对非酒精性脂肪肝Th17细胞及相关细胞因子的影响*
Rev Assoc Med Bras A comparison of IL-17 and IL-34 concentrations in the cerebrospinal fluid of patients with acute inflammatory demyelinating neuropathy and chronic?¡­ 33295414
BEHAVIOURAL BRAIN RESEARCH Minocycline alleviates Gulf War Illness rats via altering gut microbiome, attenuating neuroinflammation and enhancing hippocampal neurogenesis 34000339
Int J Clin Pract Serum Galectin©\3 Levels in Patients with Psoriasis 34137138
cells The Effect of the Clenbuterol¡ª¦Â2-Adrenergic Receptor Agonist on the Peripheral Blood Mononuclear Cells Proliferation, Phenotype, Functions, and Reactive Oxygen?¡­ 33920705
Mediators of Inflammation Sparstolonin B Exerts Therapeutic Effects on Collagen-Induced Arthritis by Inhibiting the NLRP3 Inflammasome and Reducing the Activity of ¦Á1, 3 ¡­
CNS Neurosci Ther Alterations in the gut microbiome with hemorrhagic transformation in experimental stroke 34591349
PLoS One Evaluation of the involvement of Th17-cells in the pathogenesis of canine spinal cord injury 34591917
J Periodontol Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study 34730850
Transl Psychiatry Associations between expression of indoleamine 2, 3-dioxygenase enzyme and inflammatory cytokines in patients with first-episode drug-naive … 34802039
Viruses Interleukin-10-Mediated Lymphopenia Caused by Acute Infection with Foot-and-Mouth Disease Virus in Mice 34960627
PLoS One Th17 cell-mediated immune response in a subpopulation of dogs with idiopathic epilepsy Pubmed:35025939
Pathogens Elevated Levels of IL-33, IL-17 and IL-25 Indicate the Progression from Chronicity to Hepatocellular Carcinoma in Hepatitis C Virus Patients Pubmed:35056005
Biomed Pharmacother Jiangu granule ameliorated OVX rats bone loss by modulating gut microbiota-SCFAs-Treg/Th17 axis Pubmed:35453007
npj Biofilms and Microbiomes The microbiota-gut-kidney axis mediates host osmoregulation in a small desert mammal Pubmed:35379849
Expert Opinion on Therapeutic Targets Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma Pubmed:35549595
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