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SKU(재고 관리 코드):SEG369Rb

ELISA Kit for ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)

ELISA Kit for ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1)

UOM
Sensitivity
Detection range

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

SEG369Rb

Organism Species

Oryctolagus cuniculus (Rabbit).

Sample Type

tissue homogenates, cell lysates and other biological fluids

Test Method

Double-antibody Sandwich

Assay Length

3h

Detection Range

0.312-20ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.117ng/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

 

Specificity

This assay has high sensitivity and excellent specificity for detection of ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1).
No significant cross-reactivity or interference between ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) and analogues was observed.

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents Quantity Reagents Quantity
Pre-coated, ready to use 96-well strip plate 1 Plate sealer for 96 wells 4
Standard 2 Standard Diluent 1×20mL
Detection Reagent A 1×120µL Assay Diluent A 1×12mL
Detection Reagent B 1×120µL Assay Diluent B 1×12mL
TMB Substrate 1×9mL Stop Solution 1×6mL
Wash Buffer (30 × concentrate) 1×20mL Instruction manual 1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
8. Add 50µL Stop Solution. Read at 450nm immediately.

Catalog No. Related products for research use of Oryctolagus cuniculus (Rabbit) Organism species Applications (RESEARCH USE ONLY!)
RPG369Rb01 Recombinant ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Positive Control; Immunogen; SDS-PAGE; WB.
PAG369Rb52 Polyclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) WB; IHC; ICC; IP.
PAG369Rb01 Polyclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) WB; IHC; ICC; IP.
PAG369Rb51 Polyclonal Antibody to ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) WB; IHC; ICC; IP.
SEG369Rb ELISA Kit for ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) Enzyme-linked immunosorbent assay for Antigen Detection.
LMG369Rb Magnetic Luminex Assay Kit for ATPase, Na+/K+ Transporting Alpha 1 Polypeptide (ATP1a1) ,etc. Magnetic Luminex Assay for Antigen Detection.
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