YWHAZ; 14-3-3 protein zeta/delta; Protein kinase C inhibitor protein 1; KCIP-1
Applications:
WB;IHC;IP;IF;ELISA
Recommended Dilutions:
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human 14-3-3 zeta. AA range:24-73
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
28kD
GeneID（Human）:
7534
Human Swiss-Prot No:
P63104
Cellular localization:
Cytoplasm . Melanosome . Located to stage I to stage IV melanosomes.
Background:
This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs. The encoded protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. Several transcript variants that differ in the 5' UTR but that encode the same protein have been identified for this gene. [provided by RefSeq, Oct 2008],
Western Blot analysis of various cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000
Western Blot analysis of COLO205 cells using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000
Western Blot analysis of 293T HELA using 14-3-3 ζ Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Immunofluorescence analysis of HeLa cells, using 14-3-3 zeta Antibody. The picture on the right is blocked with the synthesized peptide.