anti- NHP2 antibody

PRODUCT INFORMATION

Product Name

NHP2 antibody

Size

100µg

Form

liquid

Purification

Immunogen affinity purified

Purity

≥95% as determined by SDS-PAGE

Host

Rabbit

Clonality

polyclonal

Isotype

IgG

Storage

PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)

BACKGROUND

Required for ribosome biogenesis and telomere maintenance. Part of the H/ACA small nucleolar ribonucleoprotein(H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine("psi") residues, which may serve to stabilize the conformation of rRNAs. May also be required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase(TERT) holoenzyme.

IMMUNOGEN INFORMATION

Immunogen

NHP2 ribonucleoprotein homolog

Synonyms

NHP2, NHP2P, NOLA2, snoRNP protein NHP2

Observed MW

17-20kd

APPLICATION

Tested Application

ELISA, WB, IHC, IF, IP

Recommended Dilution

WB: 1:500-1:1000; IP: 1:500-1:1000; IHC: 1:20-1:200; IF: 1:10-1:100

UNIPROT INFORMATION

UniProt ID

Q9NX24

IMAGES

Immunofluorescent analysis of HepG2 cells using FNab05727 (NPH2 Antibody) at dilution of 1:25

IP result of anti-NHP2(IP: FNab05727, 4μg; Detection: FNab05727 1:1000 ) with HepG2 cells lysate 3600μg.

Hela cells were subjected to SDS PAGE followed by western blot with FNab05727(NHP2 antibody) at dilution of 1:1000