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Biospes
SKU(재고 관리 코드):BEK1345
Human B7-1/CD80 ELISA Kit
Human B7-1/CD80 ELISA Kit
Application: For quantitative detection of B7-1 / CD80 in human serum, plasma, body fluids, tissue lysatesor cell culture supernatants.
Introduction: The Cluster of differentiation 80 (also CD80 and B7-1) is a B7, type I membrane proteinin the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is closely related to CD86, another B7 protein (B7-2), and often works in tandem. Both CD80 and CD86 interact with costimulatory receptors CD28, CTLA-4 (CD152) and the p75 neurotrophin receptor.CD80 is a member of the B7 family, which consists of molecules present at APCs and their receptors present on the T-cells.[7] CD80 is present specifically on DC, activated B-cells, and macrophages, but also T-cells. CD80 is also a transmembrane glycoprotein and a member of the Ig superfamily.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-B7-1 / CD80 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-1 / CD80 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theB7-1 / CD80amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofB7-1 / CD80can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <12 pg/ml
Introduction: The Cluster of differentiation 80 (also CD80 and B7-1) is a B7, type I membrane proteinin the immunoglobulin superfamily, with an extracellular immunoglobulin constant-like domain and a variable-like domain required for receptor binding. It is closely related to CD86, another B7 protein (B7-2), and often works in tandem. Both CD80 and CD86 interact with costimulatory receptors CD28, CTLA-4 (CD152) and the p75 neurotrophin receptor.CD80 is a member of the B7 family, which consists of molecules present at APCs and their receptors present on the T-cells.[7] CD80 is present specifically on DC, activated B-cells, and macrophages, but also T-cells. CD80 is also a transmembrane glycoprotein and a member of the Ig superfamily.
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-B7-1 / CD80 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-1 / CD80 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theB7-1 / CD80amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofB7-1 / CD80can be calculated.
Storage and Expiration: Store at 2-8℃ for 6 months, or at -20℃ for 12 months.
Range: 62.5 pg/ml - 4000 pg/ml
Sensitivity: <12 pg/ml
