Cloud-clone
SKU(재고 관리 코드):LAP549Mu81
FITC-Linked Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
FITC-Linked Polyclonal Antibody to Wingless Type MMTV Integration Site Family, Member 5A (WNT5A)
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
Product No.
LAP549Mu81
Organism Species
Mus musculus (Mouse).
Source
Antibody labeling
Ig Type
IgG
Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Label
FITC
Original Antibody
n/a
Buffer Formulation
PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Traits
Liquid
Concentration
500µg/mL
Organism Species More
n/a
Applications
WB; IHC; ICC; IF.
If the antibody is used in flow cytometry, please check FCM antibodies.
UOM
20µl 100µl 200µl 1ml 10ml
SPECIFITY
The antibody is a rabbit polyclonal antibody raised against WNT5A. It has been selected for its ability to recognize WNT5A in immunohistochemical staining and western blotting.
USAGE
Western blotting: 0.2-2µg/mL;1:250-2500
Immunohistochemistry: 5-20µg/mL;1:25-100
Immunocytochemistry: 5-20µg/mL;1:25-100
Optimal working dilutions must be determined by end user.
STORAGE
Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
STABILITY
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
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Magazine |
Citations |
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Liver International |
Secreted frizzled-related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis Pubmed:25488180 |
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Journal of Physiology and Biochemistry |
Wnt5a reverses the inhibitory effect of hyperoxia on transdifferentiation of alveolar epithelial type II cells to type I cells PubMed: 26547443 |
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Liver |
Secreted frizzled‐related protein 5 (Sfrp5) decreases hepatic stellate cell activation and liver fibrosis PubMed: 25488180 |
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Transl Res |
Wnt5a is expressed in spondyloarthritis and exerts opposite effects on enthesis and bone in murine organ and cell cultures PubMed: 26163991 |
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不确定杂志名 |
Untersuchungen der Serum-Konzentrationen des proinflammatorischen Glykoproteins wnt-5a und seines antiinflammatorischen Inhibitors sFRP-5 bei an … servlets:MCRFileNodeServlet |
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Life Sciences |
Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. pubmed:28754618 |
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Arteriosclerosis, Thrombosis, and Vascular Biology |
Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With … Pubmed:29674475 |
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Stem Cells |
Reduced Cell Division Control Protein 42 Activity Compromises Hematopoiesis‐Supportive Function of Fanconi Anemia Mesenchymal Stromal Cells Pubmed:29377497 |
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Science Translational Medicine |
Adipose tissue–derived WNT5A regulates vascular redox signaling in obesity via USP17/RAC1-mediated activation of NADPH oxidases Pubmed: 31534019 |
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J Clin Endocrinol Metab |
Role of wnt5a in Metabolic Inflammation in Humans Pubmed: 30137542 |
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Cancer Research |
Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis Pubmed: 31932454 |
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CLINICAL BIOCHEMISTRY |
Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease Pubmed: 33188773 |
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Sao Paulo Medical Journal |
Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … Pubmed: 32491086 |
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Biomaterials |
An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties 34823885 |
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PLoS One |
Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study Pubmed:34990470 |
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J Clin Periodontol |
Association between bone turnover markers and periodontitis: A population‐based cross‐sectional study Pubmed:35569034 |
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