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ELK Biotechnology

ELK9548

Guinea pig AngII(Angiotensin II) ELISA Kit

Guinea pig AngII(Angiotensin II) ELISA Kit

Sensitivity

Guinea pig AngII(Angiotensin II) ELISA Kit

Overview

Product name: Guinea pig AngII(Angiotensin II) ELISA Kit
Reactivity: Guinea pig
Alternative Names: Ang-II; Angiotensin-2
Assay Type: Sandwich
Sensitivity: 2.15 pg/mL
Standard: 400 pg/mL
Detection Range: 6.25-400 pg/mL
Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Length: 3.5h
Research Area: Endocrinology;Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Guinea pig AngII. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Guinea pig AngII. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Guinea pig AngII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Guinea pig AngII in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
400.00 2.268 2.185
200.00 1.686 1.603
100.00 1.251 1.168
50.00 0.882 0.799
25.00 0.539 0.456
12.50 0.377 0.294
6.25 0.236 0.153
0.00 0.083 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant AngII and the recovery rates were calculated by comparing the measured value to the expected amount of AngII in samples.
Matrix Recovery range Average
serum(n=5) 84-97% 90%
EDTA plasma(n=5) 80-95% 87%
Heparin plasma(n=5) 78-91% 84%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of AngII and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 88-95% 87-98% 86-97% 90-101%
EDTA plasma(n=5) 93-101% 79-96% 85-99% 79-93%
Heparin plasma(n=5) 81-96% 85-96% 87-98% 95-102%
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