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ELK Biotechnology

ELK9455

Horse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Horse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Sensitivity

Horse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit

Overview

Product name: Horse cTnT/TNNT2(Troponin T Type 2, Cardiac) ELISA Kit
Reactivity: Horse
Alternative Names: CMH2; cTnT; TnTc; Cardiomyopathy,Hypertrophic 2; Cardiac muscle troponin T; TNNT2; Troponin T Type 2, Cardiac
Assay Type: Sandwich
Sensitivity: 6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse cTnT/TNNT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse cTnT/TNNT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse cTnT/TNNT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse cTnT/TNNT2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.179 2.097
500.00 1.612 1.530
250.00 1.273 1.191
125.00 0.897 0.815
62.50 0.567 0.485
31.25 0.369 0.287
15.63 0.206 0.124
0.00 0.082 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant cTnT/TNNT2 and the recovery rates were calculated by comparing the measured value to the expected amount of cTnT/TNNT2 in samples.
Matrix Recovery range Average
serum(n=5) 90-105% 97%
EDTA plasma(n=5) 82-97% 89%
Heparin plasma(n=5) 84-98% 91%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of cTnT/TNNT2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 90-101% 92-102% 81-93% 86-98%
EDTA plasma(n=5) 84-98% 95-103% 86-98% 89-98%
Heparin plasma(n=5) 95-103% 86-93% 91-96% 87-98%
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