FineTest
SKU(재고 관리 코드):EH2094
Human FECH(Ferrochelatase, mitochondrial) ELISA Kit
Human FECH(Ferrochelatase, mitochondrial) ELISA Kit
Product Name
Human FECH(Ferrochelatase, mitochondrial) ELISA Kit
Alias
FECH ELISA Kit, Heme synthase ELISA Kit, Protoheme ferro-lyase ELISA Kit
Detection Method
Sandwich ELISA, Double Antibody
Application
FECH ELISA Kit allows for the in vitro quantitative determination of FECH concentrations in serum, plasma, tissue homogenates and other biological fluids.
Size
96T
Range
0.156-10ng/ml
Sensitivity
< 0.094ng/ml
Species
Human
UniProt ID
Standard Curve
Storage
2-8 ℃ for 6 months
Recovery
Matrices listed below were spiked with certain level of FECH and the recovery rates were calculated by comparing the measured value to the expected amount of FECH in samples.
Matrix |
Recovery range(%) |
Average(%) |
serum(n=5) |
86-103 |
96 |
EDTA plasma(n=5) |
91-103 |
97 |
heparin plasma(n=5) |
88-104 |
95 |
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FECH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample |
1:2 |
1:4 |
1:8 |
serum(n=5) |
88-102% |
85-101% |
85-105% |
EDTA plasma(n=5) |
82-100% |
84-95% |
85-96% |
heparin plasma(n=5) |
85-100% |
81-96% |
82-96% |
CV(%)
Intra-Assay: CV<8%
Inter-Assay: CV<10%
Note
For Research Use Only