Human Kynurenine (KYN)ELISA Kit

For the quantitative determination of endogenic human kynurenine (KYN)
concentrations in serum, urine, tissue homogenates.

PRINCIPLE OF THE ASSAY
This assay employs the competitive inhibition enzyme immunoassay technique.
The microtiter plate provided in this kit has been pre-coated with an antigen.
Standards or samples are added to the appropriate microtiter plate wells with
antibody specific for KYN and Horseradish Peroxidase (HRP) conjugated
goat-anti-rabbit antibody. The competitive inhibition reaction is launched
between with pre-coated KYN and KYN in samples. A substrate solution is
added to the wells and the color develops in opposite to the amount of KYN in
the sample. The color development is stopped and the intensity of the color is
measured.

DETECTION RANGE
7.8 pmol/ml-500 pmol/ml.

SENSITIVITY
The minimum detectable dose of human KYN is typically less than 3.9 pmol/ml.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as
the lowest human KYN concentration that could be differentiated from zero.

SPECIFICITY
This assay has high sensitivity and excellent specificity for detection of human
KYN. No significant cross-reactivity or interference between human KYN and
analogues was observed.

Note: Limited by current skills and knowledge, it is impossible for us to complete
the cross-reactivity detection between human KYN and all the analogues,
therefore, cross reaction may still exist.

PRECISION
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to
assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

LIMITATIONS OF THE PROCEDURE

• FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTICPROCEDURES.
• The kit should not be used beyond the expiration date on the kit label.
• Do not mix or substitute reagents with those from other lots or sources.
• If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
• Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding.
• This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.