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ELK Biotechnology

ELK9445

Human sTNFRSF1A(Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit

Human sTNFRSF1A(Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit

Sensitivity

Human sTNFRSF1A(Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit

Overview

Product name: Human sTNFRSF1A(Soluble Tumor Necrosis Factor Receptor Superfamily, Member 1A) ELISA Kit
Reactivity: Human
Alternative Names: CD120A; P55; TBP1; FPF; TNF-R; TNF-R-I; TNF-R55; TNFAR; TNFR1; TNFR55; TNFR60; P55-R; P60; Tumor necrosis factor receptor 1; Tumor necrosis factor-binding protein 1
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, urine, cell lysates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;CD & Adhesion molecule;Apoptosis;
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sTNFRSF1A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sTNFRSF1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sTNFRSF1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sTNFRSF1A in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 1.987 1.898
500.00 1.515 1.426
250.00 1.106 1.017
125.00 0.828 0.739
62.50 0.589 0.500
31.25 0.377 0.288
15.63 0.226 0.137
0.00 0.089 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant sTNFRSF1A and the recovery rates were calculated by comparing the measured value to the expected amount of sTNFRSF1A in samples.
Matrix Recovery range Average
serum(n=5) 87-99% 93%
EDTA plasma(n=5) 78-96% 87%
Heparin plasma(n=5) 82-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of sTNFRSF1A and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-101% 96-105% 89-103% 95-103%
EDTA plasma(n=5) 93-102% 89-97% 85-94% 93-102%
Heparin plasma(n=5) 93-106% 86-93% 91-105% 87-98%
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