Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen:
The antiserum was produced against synthesized peptide derived from human AGPAT9. AA range:381-430
Storage:
Rabbit
Storage:
-20°C/1 year
Clonality:
Polyclonal
Isotype:
IgG
Concentration:
1 mg/ml
Observed Band:
48kD
GeneID（Human）:
84803
Human Swiss-Prot No:
Q53EU6
Cellular localization:
Endoplasmic reticulum membrane ; Multi-pass membrane protein .
Background:
This gene encodes a member of the lysophosphatidic acid acyltransferase protein family. The encoded protein is an enzyme which catalyzes the conversion of glycerol-3-phosphate to lysophosphatidic acid in the synthesis of triacylglycerol. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2012],
Western Blot analysis of HEPG2 using LPAAT-θ Polyclonal Antibody diluted at 1:1000
Western blot analysis of SH-SY5Y 3T3 lysis using LPAAT-θ antibody. Antibody was diluted at 1:1000
Immunofluorescence analysis of HepG2 cells, using PLCH Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from Jurkat cells, COLO205 cells, HeLa cells, and HUVEC cells, using PLCH Antibody. The lane on the right is blocked with the synthesized peptide.