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ELK Biotechnology

ELK9759

Mouse ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

Mouse ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

Sensitivity

Mouse ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit

Overview

Product name: Mouse ACA-IgG(Anti-Cardiolipin Antibody IgG) ELISA Kit
Reactivity: Mouse
Alternative Names: ACA-IgG; Anti-Cardiolipin Antibody IgG
Assay Type: Sandwich
Sensitivity: 1.032 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Area: Cardiovascular biology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse ACA-IgG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse ACA-IgG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse ACA-IgG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse ACA-IgG in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
200.00 2.079 1.993
100.00 1.587 1.501
50.00 1.228 1.142
25.00 0.871 0.785
12.50 0.593 0.507
6.25 0.321 0.235
3.13 0.213 0.127
0.00 0.086 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant ACA-IgG and the recovery rates were calculated by comparing the measured value to the expected amount of ACA-IgG in samples.
Matrix Recovery range Average
serum(n=5) 92-107% 99%
EDTA plasma(n=5) 89-103% 96%
Heparin plasma(n=5) 85-101% 93%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of ACA-IgG and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 87-98% 85-96% 80-96% 84-98%
EDTA plasma(n=5) 91-97% 81-93% 90-99% 81-97%
Heparin plasma(n=5) 85-96% 91-101% 87-98% 95-104%
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