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ELK Biotechnology

ELK9526

Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit

Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit

Sensitivity

Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit

Overview

Product name: Mouse BCAT1(Branched-chain-amino-acid aminotransferase, cytosolic) ELISA Kit
Reactivity: Mouse
Alternative Names: Branched-chain-amino-acid aminotransferase, cytosolic; BCAT（c）; Protein ECA39; Eca39; Bcat1
Assay Type: Sandwich
Sensitivity: 6.5 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Area: Signal transduction;Metabolic pathway
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BCAT1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BCAT1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BCAT1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BCAT1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Standard curve

Concentration (ng/mL) OD Corrected OD
1000.00 2.079 1.992
500.00 1.572 1.485
250.00 1.140 1.053
125.00 0.882 0.795
62.50 0.593 0.506
31.25 0.361 0.274
15.63 0.235 0.148
0.00 0.087 0.000

Precision

Intra-assay Precision (Precision within an assay)：CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-assay Precision (Precision between assays)：CV%<10%

Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Recovery

Matrices listed below were spiked with certain level of recombinant BCAT1 and the recovery rates were calculated by comparing the measured value to the expected amount of BCAT1 in samples.
Matrix Recovery range Average
serum(n=5) 94-107% 100%
EDTA plasma(n=5) 82-98% 90%
Heparin plasma(n=5) 81-95% 88%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of BCAT1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Matrix 1:2 1:4 1:8 1:16
serum(n=5) 89-97% 85-94% 89-96% 90-99%
EDTA plasma(n=5) 85-92% 89-103% 97-105% 89-103%
Heparin plasma(n=5) 81-96% 85-92% 82-96% 91-102%
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