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SKU(재고 관리 코드):LMB409Po
Magnetic Luminex Assay Kit for Hemoglobin (HB) ,etc.
Magnetic Luminex Assay Kit for Hemoglobin (HB) ,etc.
Product No.
LMB409Po
Organism Species
Sus scrofa; Porcine (Pig).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Competitive Inhibition
Assay Length
1.5h
Detection Range
0.29-300ug/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.097 ug/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Hemoglobin (HB) ,etc..
No significant cross-reactivity or interference between Hemoglobin (HB) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Hemoglobin (HB) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Hemoglobin (HB) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 83-94 | 86 |
| EDTA plasma(n=5) | 78-98 | 91 |
| heparin plasma(n=5) | 80-103 | 85 |
| sodium citrate plasma(n=5) | 80-91 | 86 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Hemoglobin (HB) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Hemoglobin (HB) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Hemoglobin (HB) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-91% | 91-101% | 86-101% | 98-105% |
| EDTA plasma(n=5) | 92-101% | 99-105% | 80-97% | 84-101% |
| heparin plasma(n=5) | 98-105% | 83-98% | 91-105% | 84-101% |
| sodium citrate plasma(n=5) | 98-105% | 79-92% | 85-96% | 90-104% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:HB) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 50μL standard or sample to each well,
add 10μL magnetic beads,and 50μL Detection Reagent A,incubate 60min at 37°C on shaker;
3. Wash plate on magnetic frame for three times;
4. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
5. Wash plate on magnetic frame for three times;
6. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology | Effects of ectoine on behavioural, physiological and biochemical parameters of Daphnia magna Pubmed:25460046 |
| PLoS One. | Comparative Proteomics Identifies Host Immune System Proteins Affected by Infection with Mycobacterium bovis Pubmed:27027307 |
| Parasites & Vectors | The intracellular bacterium Anaplasma phagocytophilum selectively manipulates the levels of vertebrate host proteins in the tick vector Ixodes scapularis. pubmed:27561965 |
| Proteomics. Clinical Applications | Elevated levels of protein AMBP in cerebrospinal fluid of women with preeclampsia compared to normotensive pregnant women. pubmed:27615121 |
| Molecular nutrition & food research | Chicory inulin ameliorates type 2 diabetes mellitus and suppresses JNK and MAPK pathways in vivo and in vitro pubmed:28105758 |
| Frontiers in Immunology | Oxidized Hemoglobin Is Antigenic and Immunogenic in Lupus pubmed:28694810 |
| 新規人工酸素運搬体HemoAct を用いた脳虚血再灌流障害治療開発に関する研究 : | |
| Acta Neuropathologica Communications | Mural cell-derived laminin-α5 plays a detrimental role in ischemic stroke Pubmed: 30777135 |
| Angiogenesis | Decylubiquinone suppresses breast cancer growth and metastasis by inhibiting angiogenesis via the ROS/p53/BAI1 signaling pathway Pubmed: 32020421 |
| Food Bioscience | Water-extractable zinc protoporphyrin IX in Parma ham predominantly exists as complexes with hemoglobin and myoglobin |
| Photochem Photobiol | Effects of Human Serum Albumin on the Fluorescence Intensity and Tumor Imaging Properties of IR‐780 Dye 34687567 |
| Catalog No. | Related products for research use of Sus scrofa; Porcine (Pig) Organism species | Applications (RESEARCH USE ONLY!) |
| NPB409Po01 | Native Hemoglobin (HB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB409Po01 | Polyclonal Antibody to Hemoglobin (HB) | WB; IHC; ICC; IP. |
| LAB409Po71 | Biotin-Linked Polyclonal Antibody to Hemoglobin (HB) | WB; IHC; ICC. |
| CEB409Po | ELISA Kit for Hemoglobin (HB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMB409Po | Magnetic Luminex Assay Kit for Hemoglobin (HB) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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