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SKU(재고 관리 코드):LMA170Hu
Magnetic Luminex Assay Kit for Activin B (ACVB) ,etc.
Magnetic Luminex Assay Kit for Activin B (ACVB) ,etc.
Product No.
LMA170Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.98-1000pg/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.327 pg/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Activin B (ACVB) ,etc..
No significant cross-reactivity or interference between Activin B (ACVB) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Activin B (ACVB) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Activin B (ACVB) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 79-101 | 90 |
| EDTA plasma(n=5) | 89-96 | 92 |
| heparin plasma(n=5) | 86-99 | 92 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Activin B (ACVB) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Activin B (ACVB) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Activin B (ACVB) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-98% | 79-104% | 93-103% | 86-101% |
| EDTA plasma(n=5) | 83-97% | 92-99% | 92-101% | 79-99% |
| heparin plasma(n=5) | 88-96% | 81-92% | 88-95% | 80-96% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:ACVB) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Gastroenterology Research and Practice | Serum Activins and Follistatin during the Treatment of Chronic Hepatitis C Genotypes 1 and 4 and Their Correlations with Viral Load and Liver Enzymes: A Preliminary Report. Pubmed: 24799891 |
| Gastroenterol Res Pract.? | Serum activins and follistatin during the treatment of chronic hepatitis c genotypes 1 and 4 and their correlations with viral load and liver enzymes: A preliminary report Pubmed:Pmc3995172 |
| Clin Exp Med.? | Effects?of?chronic hepatitis C?genotype?1?and 4 on serum activins and follistatin in treatment na?ve patients and their correlations with interleukin-6, tumour necrosis factor-α, viral load and liver damage. Pubmed:24925642 |
| Clin Biochem | Serum activin B concentration as predictive biomarker for ectopic pregnancy Pubmed:26968108 |
| BMC Cancer. | Activins and their related proteins in colon carcinogenesis: insights from early and advanced azoxymethane rat models of colon cancer. pubmed:27835986 |
| Australasian Journal of Ultrasound in Medicine | The potential value of activin B and fibronectin for the triage of pregnancies of unknown location and prediction of first trimester viability 10.1002:ajum.12090 |
| Pathology & Oncology Research | Profiling Activins and Follistatin in Colorectal Cancer According to Clinical Stage, Tumour Sidedness and Smad4 Status 34867090 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| SEA170Hu | ELISA Kit for Activin B (ACVB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA170Hu | Magnetic Luminex Assay Kit for Activin B (ACVB) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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