Cloud-clone
SKU(재고 관리 코드):LMA012Ra
Magnetic Luminex Assay Kit for S100 Calcium Binding Protein (S100) ,etc.
Magnetic Luminex Assay Kit for S100 Calcium Binding Protein (S100) ,etc.
Product No.
LMA012Ra
Organism Species
Rattus norvegicus (Rat).
Sample Type
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of S100 Calcium Binding Protein (S100) ,etc..
No significant cross-reactivity or interference between S100 Calcium Binding Protein (S100) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant S100 Calcium Binding Protein (S100) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of S100 Calcium Binding Protein (S100) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 81-91 | 88 |
| EDTA plasma(n=5) | 80-95 | 91 |
| heparin plasma(n=5) | 91-98 | 95 |
| sodium citrate plasma(n=5) | 85-103 | 99 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level S100 Calcium Binding Protein (S100) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level S100 Calcium Binding Protein (S100) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of S100 Calcium Binding Protein (S100) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-99% | 95-102% | 87-101% | 86-97% |
| EDTA plasma(n=5) | 82-97% | 80-102% | 82-91% | 91-104% |
| heparin plasma(n=5) | 79-91% | 88-101% | 87-103% | 83-102% |
| sodium citrate plasma(n=5) | 96-105% | 93-104% | 94-101% | 89-97% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:S100) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| Brain Research | Vasoactive intestinal peptide protects against ischemic brain damage induced by focal cerebral ischemia in rats ScienceDirect: S0006899311008663 |
| J Exp Neurosci | A New Technique for Collection of Cerebrospinal Fluid in Rat Pups PubMed: 26056488 |
| PLoS One | Regulation of Nutritional Metabolism in Transition Dairy Cows: Energy Homeostasis and Health in Response to Post-Ruminal Choline and Methionine pubmed:27501393 |
| brain research | Ellagic acid exerts protective effect in intrastriatal 6-hydroxydopamine rat model of Parkinson's disease: Possible involvement of ERβ/Nrf2/HO-1 signaling. pubmed:28238669 |
| КиберЛенинка | Levels of neurotropic autoantibodies in patients with schizophrenia article:n |
| Biomed Research International | Epigallocatechin-3-Gallate Reduces Neuronal Apoptosis in Rats after Middle Cerebral Artery Occlusion Injury via PI3K/AKT/eNOS Signaling Pathway Pubmed:29770336 |
| Metabolic Brain Disease | Trigonelline protects hippocampus against intracerebral Aβ (1–40) as a model of Alzheimer's disease in the rat: insights into underlying mechanisms Pubmed: 30421246 |
| ВОЗМОЖНОСТЬ ПРОГНОЗИРОВАНИЯ ОСТРОЙ НЕСПЕЦИФИЧЕСКОЙ БОЛИ В НИЖНЕЙ ЧАСТИ СПИНЫ С ИСПОЛЬЗОВАНИЕМ СЫВОРОТОЧНЫХ … | |
| ВЛИЯНИЕ ХРОНИЧЕСКОГО ВВЕДЕНИЯ НЕЙРОЛЕПТИКОВ НА УРОВНИ НЕЙРОСПЕЦИФИЧЕСКИХ АУТОАНТИТЕЛ В КРОВИ У КРЫС |
Share
