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SKU(재고 관리 코드):LMA600Hu
Magnetic Luminex Assay Kit for Granzyme B (GZMB) ,etc.
Magnetic Luminex Assay Kit for Granzyme B (GZMB) ,etc.
Product No.
LMA600Hu
Organism Species
Homo sapiens (Human).
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test Method
Double-antibody Sandwich
Assay Length
3.5h
Detection Range
0.01-10ng/mL
Sensitivity
The minimum detectable dose of this kit is typically less than 0.003 ng/mL.
UOM
1Plex 2Plex 3Plex 4Plex 5Plex 6Plex 7Plex 8Plex
Specificity
This assay has high sensitivity and excellent specificity for detection of Granzyme B (GZMB) ,etc..
No significant cross-reactivity or interference between Granzyme B (GZMB) ,etc. and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Granzyme B (GZMB) ,etc. and the recovery rates were calculated by comparing the measured value to the expected amount of Granzyme B (GZMB) ,etc. in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 97-104 | 101 |
| EDTA plasma(n=5) | 92-99 | 96 |
| heparin plasma(n=5) | 90-97 | 93 |
| sodium citrate plasma(n=5) | 81-91 | 87 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Granzyme B (GZMB) ,etc. were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Granzyme B (GZMB) ,etc. were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Granzyme B (GZMB) ,etc. and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 80-105% | 90-98% | 96-104% | 83-98% |
| EDTA plasma(n=5) | 78-101% | 82-96% | 92-105% | 78-94% |
| heparin plasma(n=5) | 80-98% | 98-105% | 92-101% | 96-105% |
| sodium citrate plasma(n=5) | 87-97% | 82-104% | 79-95% | 94-102% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| 96-well plate | 1 | Plate sealer for 96 wells | 4 |
| Pre-Mixed Standard | 2 | Standard Diluent | 1×20mL |
| Pre-Mixed Magnetic beads (22#:GZMB) | 1 | Analysis buffer | 1×20mL |
| Pre-Mixed Detection Reagent A | 1×120μL | Assay Diluent A | 1×12mL |
| Detection Reagent B (PE-SA) | 1×120μL | Assay Diluent B | 1×12mL |
| Sheath Fluid | 1×10mL | Wash Buffer (30 × concentrate) | 1×20mL |
| Instruction manual | 1 |
Assay procedure summary
1. Preparation of standards, reagents and samples before the experiment;
2. Add 100μL standard or sample to each well,
add 10μL magnetic beads, and incubate 90min at 37°C on shaker;
3. Remove liquid on magnetic frame, add 100μL prepared Detection Reagent A. Incubate 60min at 37°C on shaker;
4. Wash plate on magnetic frame for three times;
5. Add 100μL prepared Detection Reagent B, and incubate 30 min at 37°C on shaker;
6. Wash plate on magnetic frame for three times;
7. Add 100μL sheath solution, swirl for 2 minutes, read on the machine.
| Magazine | Citations |
| International Journal of Cancer | Effective immunotherapy of rat glioblastoma with prolonged intratumoral delivery of exogenous heat shock protein Hsp70 Pubmed:24691976 |
| Biol Pharm Bull. | Z-100, an Immunomodulatory Extract of Mycobacterium tuberculosis Strain Aoyama B, Prevents Spontaneous Lymphatic Metastasis of B16-BL6 Melanoma Pubmed:24818259 |
| Journal of Dermatological Science | Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopicdermatitis. pubmed:27686401 |
| Oncotarget | Tumor-derived granzyme B-expressing neutrophils acquire antitumor potential after lipid A treatment Pubmed:29983866 |
| Theranostics | A novel Granzyme B nanoparticle delivery system simulates immune cell functions for suppression of solid tumors Pubmed: 31695790 |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma 34377237 |
| Catalog No. | Related products for research use of Homo sapiens (Human) Organism species | Applications (RESEARCH USE ONLY!) |
| RPA600Hu01 | Recombinant Granzyme B (GZMB) | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA600Hu01 | Polyclonal Antibody to Granzyme B (GZMB) | WB; IHC |
| MAA600Hu22 | Monoclonal Antibody to Granzyme B (GZMB) | WB |
| MAA600Hu21 | Monoclonal Antibody to Granzyme B (GZMB) | WB |
| SEA600Hu | ELISA Kit for Granzyme B (GZMB) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA600Hu | Magnetic Luminex Assay Kit for Granzyme B (GZMB) ,etc. | Magnetic Luminex Assay for Antigen Detection. |
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