Cloud-clone

SKU(재고 관리 코드):CEA632Hu

ELISA Kit for Pepsin (PP)

Name: ELISA Kit for Pepsin (PP)
Brand: Cloud-clone
SKU: CEA632Hu
Price: 665.0 KRW
Availability: InStock

UOM 96T

Sensitivity The minimum detectable dose of this kit is typically less than 0.93ng/mL

Detection range 2.47-200ng/mL

제품 이형 상품

수량

Enzyme-linked immunosorbent assay for Antigen Detection.

Product No.

CEA632Hu

Organism Species

Homo sapiens (Human).

Sample Type

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Test Method

Competitive Inhibition

Assay Length

Detection Range

2.47-200ng/mL

Sensitivity

The minimum detectable dose of this kit is typically less than 0.87ng/mL.

UOM

48T 96T 96T*5 96T*10 96T*100

Specificity

This assay has high sensitivity and excellent specificity for detection of Pepsin (PP).
No significant cross-reactivity or interference between Pepsin (PP) and analogues was observed.

Recovery

Matrices listed below were spiked with certain level of recombinant Pepsin (PP) and the recovery rates were calculated by comparing the measured value to the expected amount of Pepsin (PP) in samples.

Matrix	Recovery range (%)	Average(%)
serum(n=5)	93-102	97
EDTA plasma(n=5)	81-99	91
heparin plasma(n=5)	88-99	94

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Pepsin (PP) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Pepsin (PP) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pepsin (PP) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8	1:16
serum(n=5)	88-99%	92-105%	86-94%	78-101%
EDTA plasma(n=5)	83-97%	82-101%	86-99%	95-102%
heparin plasma(n=5)	97-105%	85-94%	97-104%	97-104%

Stability

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Reagents and materials provided

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120µL	Assay Diluent A	1×12mL
Detection Reagent B	1×120µL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

Assay procedure summary

1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 3 times;
4. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C;
5. Aspirate and wash 5 times;
6. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
7. Add 50µL Stop Solution. Read at 450 nm immediately.